ThermoFisher Scientific QuantStudio 7 Pro User Manual

For Research Use Only. Not for use in diagnostic procedures.
QuantStudio
™
6 Pro and 7 Pro Real-Time PCR
Systems
USER GUIDE
Publication Number MAN0018045
Revision E.0

ThermoFisher Scientific QuantStudio 7 Pro Specifications

General

Instrument Type	Real-Time PCR System
Number of Channels	6 channels
Temperature Uniformity	±0.4°C
Thermal accuracy	±0.25°C
Detection	CCD camera
Data Collection	Real-time
Multiplexing capability	Up to 6 targets
Thermal Cycler Block	96-well
Supported chemistries	TaqMan, SYBR Green
Reaction volume range	10–100 μL
Temperature Range	4°C to 100°C
Thermal range	4°C to 100°C
Optical System	LED-based
Light Source	LED
Software	QuantStudio Design & Analysis Software
Connectivity	Ethernet, USB
Power Requirements	100-240 V, 50/60 Hz
Excitation/Emission filters	Configurable filter sets

Summary

CHAPTER 1 Product information

Network and password security requirements

Discusses network configuration and password policies for system security.

Instrument hardware description

Software description

CHAPTER 2 Get started

Precautions for use

Lists critical safety warnings and precautions for instrument operation.

CHAPTER 3 Create and run plates on the instrument

Options to run a plate

Details the steps for creating and initiating a run on the instrument.

CHAPTER 5 Options for a run

View real-time data and plots during a run

CHAPTER 7 Calibrate and verify instrument performance

Instrument and block calibration

Explains the calibration process for the instrument and its blocks.

Calibration and verification schedule

Perform ROI/uniformity, background, and dye calibrations

Details the procedures for performing ROI, background, and dye calibrations.

Perform verification using RNase P plates

Describes how to perform verification using RNase P plates.

CHAPTER 8 Manage profiles

Link the instrument to your Connect account

CHAPTER 9 Use the instrument with the Security, Auditing, and E-signature (SAE) v2.0 module

Security, Auditing, and E-signature (SAE) v2.0 module functions

Enable SAE functions

Provides a workflow for enabling SAE functions across system components.

CHAPTER 10 Maintain the instrument

Install a block

Change the block

Explains how to replace the instrument block and heated cover.

Decontaminate the sample block

Provides procedures for cleaning and decontaminating the sample block.

CHAPTER 11 Configure the instrument settings

Configure the instrument settings

Covers various instrument settings configuration options.

Configure the network

Details the steps for configuring the instrument's network settings.

Configure maintenance and service settings

Request technical support with Smart Help

Details how to request technical support using the Smart Help feature.

APPENDIX B Connect the instrument to a network

Connect the instrument to a wired network

Connect the instrument to a wireless network

Download and install the desktop software

Guides downloading and installing the QuantStudio desktop software.

Networking

APPENDIX D Safety

Instrument safety

Covers general safety precautions related to the instrument itself.

Physical injury

Warns about potential physical injuries, including those from moving parts.

Electrical safety

Outlines critical safety measures related to electrical supply and components.

APPENDIX E Documentation and support

Customer and technical support

Provides information on how to contact customer and technical support.