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ThermoFisher Scientific QuantStudio 5 Real-Time PCR User Manual

ThermoFisher Scientific QuantStudio 5 Real-Time PCR
80 pages
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For Research, Forensic, or Paternity Use Only. For licensing and limited use restrictions
visit thermofisher.com/HIDlicensing.
QuantStudio
5 Real-Time PCR Instrument
(for Human Identification)
USER GUIDE
Installation, maintenance, and administration
for use with:
HID RealTime PCR Analysis Software v1.3
Publication Number MAN0017162
Revision A.0

Table of Contents

Questions and Answers:

ThermoFisher Scientific QuantStudio 5 Real-Time PCR Specifications

General IconGeneral
TechnologyReal-Time PCR
Number of Channels5
Detection MethodFluorescence
Excitation SourcesLEDs
Temperature Uniformity±0.5°C
SoftwareQuantStudio Design and Analysis Software
ConnectivityUSB, Ethernet
Power Requirements100-240 VAC, 50/60 Hz
Supported ApplicationsGene Expression, Genotyping, Copy Number Variation, Mutation Detection, MicroRNA Analysis
Sample Capacity96-well, 384-well
Detection ChannelsFAM, VIC, ROX, Cy5
Temperature Range4°C to 100°C
Temperature Accuracy±0.25°C
Reaction Volume Range10-100 μL
Block Format96-well
ThroughputUp to 384 samples
Data Analysis SoftwareQuantStudio Software

Summary

Chapter 1 Product Information

Instrument Overview

Details the QuantStudioTM 5 Real-Time PCR Instrument's use of fluorescence-based PCR.

Installation, Verification, and Calibration

Outlines the process for installing, verifying, and calibrating the instrument.

Instrument Filters and Supported Dyes

Lists the instrument's optical filter set and compatible dyes.

Chapter 2 Install the Instrument

Workflow: Install the Instrument

Provides a step-by-step workflow for instrument installation.

Unpack and Install the Instrument

Details procedures for unpacking and physically installing the instrument.

Instrument and Computer Connections

Illustrates the physical connections between the instrument and computer.

Chapter 3 General Procedures to Use the Instrument

Precautions for Use

Lists essential safety precautions before operating the instrument.

Power On the Instrument

Step-by-step guide to powering on the QuantStudioTM 5 instrument.

Sign In

Instructions on how to sign into an instrument profile.

Chapter 4 Configure the Instrument and Manage Instrument Profiles

Initial Start Up

Guides through the initial setup tasks after powering on the instrument.

Overview of Instrument Settings (Administrator)

Details instrument settings accessible to administrator profiles.

Manage Instrument Profiles

Explains how to create, edit, and manage user profiles on the instrument.

Chapter 5 Calibrate and Verify Instrument Performance

Calibration and Verification Schedule

Recommends frequencies for performing instrument calibrations.

Perform ROI/Uniformity, Background, and Dye Calibrations

Describes how to perform key instrument calibration procedures.

Calibrate Dyes for HID-Validated Workflows

Guides on calibrating custom dyes for specific workflows.

Perform Instrument Verification Using RNase P Plates

Details the process for verifying instrument performance with RNase P plates.

Chapter 6 Maintain the Instrument

Backup or Restore the Instrument

Procedures for backing up and restoring instrument data.

Decontaminate the Sample Block

Steps to eliminate contaminants from the instrument's sample block.

APPENDIX A Troubleshooting

Instrument Troubleshooting

Provides solutions for common instrument operational issues.

Troubleshoot Calibration Failure

Offers guidance on resolving calibration failures.

APPENDIX B Parts and Materials

Consumables (96-Well, 0.2-mL Format)

Lists available consumables for the instrument, including quantities.

APPENDIX C Instrument Specification and Layout

Electrical Requirements

Specifies the electrical power and connection requirements for the instrument.

APPENDIX D Safety

Safety Alerts on this Instrument

Highlights important safety warnings found on the instrument.

Instrument Safety

Covers general safety practices and warnings related to instrument operation.

Chemical Safety

Provides guidance on safe handling of chemicals used with the instrument.

Documentation and Support

Customer and Technical Support

Information on how to contact support for assistance.

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