ThermoFisher Scientific QuantStudio 3 - Default Chapter; Table of Contents

Contents
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CHAPTER 1 About the software ........................................ 7
Compatible instruments .......................................................... 7
Compatible data les ............................................................ 7
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CHAPTER 2 Workow ................................................... 9
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CHAPTER 3 Set up a plate le ........................................ 10
Select a system template or existing plate le to set up a new plate le ............... 10
Conrm or edit run method ...................................................... 12
Run method elements ...................................................... 12
Apply the recommended run method for your master mix ....................... 12
Edit temperature ramp rate, temperature, and time length for a step ............. 13
Add or remove a step ...................................................... 13
Add or remove a stage ..................................................... 13
Add, edit, or remove a pause cycle in a PCR stage ............................. 14
Edit the ramp increment for the melt curve dissociation step .................... 15
Enable, edit, or disable Auto Delta ........................................... 15
Enable, edit, or disable VeriFlex
™
zones ...................................... 16
Conrm or edit lter settings ................................................ 16
Conrm or edit plate setup ...................................................... 17
Select plate wells .......................................................... 17
Add samples and assign to plate wells ....................................... 17
Add targets or SNP assays and assign to plate wells ........................... 21
Edit reagent information .................................................... 25
Select a passive reference .................................................. 25
Set up the standard curve .................................................. 26
Add biogroups and assign samples .......................................... 27
Edit plate le or data le information .............................................. 27
Review the plate le and save ................................................... 28
Export a Plate Setup le ........................................................ 28
QuantStudio
™
Design and Analysis v2 User Guide
3