ThermoFisher Scientific QuantStudio 3 - Page 4

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CHAPTER 4 Review and analyze data ................................ 29
W
orkow: General procedures to review the primary analysis results ................. 29
Open a data le ................................................................ 30
Review results in the Amplication Plot ........................................... 30
Evaluate the overall shape of the curves in the Amplification Plot ................ 30
Review the amplication status for each well .................................. 31
Review or edit threshold settings in the Amplication Plot ....................... 31
Review or edit baseline settings in the Amplication Plot ........................ 33
Optimize display of negative controls in the Amplication Plot ................... 33
Identify and omit outliers from analysis ........................................... 34
Review results in the Multicomponent Plot ........................................ 35
Review results in the Raw Data Plot .............................................. 36
Review results the Melt Curve Plot ............................................... 36
Review QC alerts in the Well Table ............................................... 37
Edit primary analysis settings .................................................... 37
View or edit C
q
settings ..................................................... 37
View or edit QC Alerts settings .............................................. 38
View or edit melt analysis settings ........................................... 39
Use the analysis settings from another le .................................... 39
View instrument calibration results ............................................... 40
Review ROI/Uniformity calibration results ..................................... 40
Review Background calibration results ....................................... 40
Review Dye calibration results ............................................... 41
Override the calibration data .................................................... 41
Perform additional analysis ...................................................... 42
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CHAPTER 5 Export results ............................................ 43
Export the Well Table ........................................................... 43
Export plate layout as an Excel
™
spreadsheet ..................................... 43
Export plate layout image ....................................................... 43
Export images of plots .......................................................... 43
Export data in the QuantStudio
™
le format ....................................... 44
Export data in the RDML format ................................................. 44
Generate a report .............................................................. 44
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CHAPTER 6 General procedures to analyze data in the
Quality Check tab ........................................................ 47
View the post-run summary ..................................................... 47
Congure the layout of the Quality Check tab ..................................... 47
Review individual well results in the Well Table .................................... 48
Contents
4
QuantStudio
™
Design and Analysis v2 User Guide