6
cuvettes with optional holders.
WAVELENGTH Knob: Select desired wavelength in nanometers (nm).
Wavelength Readout Window: Display desired wavelength.
Basic Operation
Sample Preparation and Analysis
A. Spectrophotometer Warm-up and 0%T Check
1. Turn on the spectrophotometer by turning on the Power Switch (IO). Allow 15
minutes for the instrument to warm up.
2. Even through the 0%T is pre-set at UNICO
®
; it may shift due to unexpected
factors like temperature change. To check 0%T, simply insert the Black Block
(item number: S-1200-111, used to check the 0%T) into the Cuvette Holder and
close the lid. Select T mode, the display should be 00.0%T. If not, press the 0%T
button to set 0%T.
3. Select either the T (Transmittance Mode) or A (Absorbance Mode) by pressing
the MODE button until the red light for T or A is on.
4. Select the desired wavelength by turning the WAVELENGTH control knob.
5. Insert the blank cuvette into Sample Compartment and close the cover.
6. Set 0.000A or 100%T with the 0A/100%T (∧) button.
7. Remove the blank square cuvette or test tube. Set it aside in case that you may
need to adjust the 0A/100%T using ∧ button later (i.e. change the wavelength).
9. Rinse a second square cuvette with a small amount of the sample solution to be
tested. Fill the square cuvette half full and wipe it.
10. Put the sample cuvette in the Sample Compartment. Close the cover.
11. Read the T or A from the Digital Display window. Remove the sample cuvette or
test tube.
Concentration Mode
C is used for determining the concentration of unknown samples.
NOTE: This method should only be used when the relationship between Absorbance
and Concentration is known to be linear. The concentration of the Standard solution
used to calibrate the instrument should be higher than the most concentrated sample.
1. Select the desired wavelength by turning the wavelength control knob.
2. Using the MODE button, select A mode.
3. Insert the cuvette containing the blank solution.
4. Set 0.000A with the ∧ button.
5. Using the MODE button, select C mode.
6. Insert a cuvette containing a standard solution of known concentration in the Sample
Compartment and set the Digital Display window to be the value of the standard by
using the ∧ and ∨ buttons.
7. Press the ENT button.
NOTE: If the reading changes, the factor required is too high (i.e. >1999) to be
displayed. In this case, divide the concentration by 10; re-select the C mode by
successive presses on the MODE button, cycling through the F, T, and A modes, and
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