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follow step 2 above to set the concentration of the standard to the reduced value.
8. With the standard concentration set, determine the concentration values of samples
with unknown concentration by inserting the sample cuvette into the Sample
Compartment and reading the value direct from the Digital Display.
9. To read the value of the multiplier used to convert Abs to Concentration, after
measuring all the samples, change the mode to F and read the multiplier from the
display. Keep a record of this value for future use.
Operational Note: if the MODE is changed to read F or A and the Concentration C
reading is “frozen”, re-start at step 1.
Factor Mode
This is a special mode for measuring concentration values of unknown samples using a
previously determined factor to convert absorbance readings to concentration.
1. After setting the wavelength, and setting zero Abs on the blank solution, using
the MODE button, select F mode.
2. Insert a cuvette containing a sample.
3. Using the ∧ and ∨ buttons, set the Digital Display window to the desired value
of the multiplier.
4. Press the ENT button. The spectrophotometer switches to the C mode.
Operational Note: If the Concentration of the sample is too high to be displayed, the
instrument will not switch to C mode when the ENT button is pressed. Dilute the sample
and multiply the concentration reading by the dilution factor to obtain the original sample
concentration. If dilution is impossible or causes other problems, you may divide the
factor value by “10” or “100” and follow step 1 to 4 to enter the “new” factor value. You
need to calculate the sample concentration by multiplying readout with the multiple “10”
or “100”.
5. Read the concentration value of the sample direct from the display.
6. Insert a cuvette containing the next sample and read the result. Repeat until all
samples have been measured.
Operational Note: if the MODE is changed to read A or T and the Concentration C
reading is “frozen”, re-start at step 1.
Maintenance
Bulb Replacement
1. Replacement bulb pn#1200-505: 6V/10W G4 with horizontal filament.
2. Turn off and unplug the instrument.
3. Remove the lamp access door at the back of the instrument by removing the four set
screws pointed by arrows on the image below. Remove the black cover next to the
access door and set it aside.
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