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Unico UV-2802 User Manual

Unico UV-2802
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Unico UV-2802 Specifications

General IconGeneral
BrandUnico
ModelUV-2802
CategoryMeasuring Instruments
LanguageEnglish

Summary

Safety and Warnings

General Use Guidelines

General guidelines for proper use by trained personnel in a laboratory.

Electrical Safety Precautions

Ensures safe operation by connecting to a properly earthed power supply.

Critical Safety Warnings

Alerts users to potential dangers from interrupted protective conductors or impaired protection.

Instrument Performance and Introduction

Performance Checks

Recommends performance checks for accuracy, especially wavelength and absorbance.

Radio Interference Compliance

Mandates the use of shielded cables for EMC compliance.

Spectrophotometer Overview

Introduces the UNICO UV-2802 as a versatile single-beam instrument.

Working Principle Explanation

Explains the fundamental components and operation of the spectrophotometer.

Instrument Operation and Setup

Spectrophotometer Block Diagram

Illustrates the relationship between the light source, monochromator, sample, detector, and display.

Unpacking and Initial Checks

Guides users through unpacking and verifying contents against the packing list.

Specifications and Installation

Packing List Details

Lists all items included with the spectrophotometer.

Technical Specifications

Provides detailed technical data including wavelength range, accuracy, and power requirements.

Installation Procedures

Step-by-step instructions for properly installing the instrument in a suitable environment.

Basic Operation and Controls

Control Panel Overview

Identifies and describes the function of each key on the control panel.

Preparing the Spectrophotometer

Steps to prepare the instrument before performing measurements.

Key Function Descriptions

Detailed explanation of the function of each key on the control panel.

Instrument Startup and Calibration

Turning On the Spectrophotometer

Guides the user through the power-on sequence and initial system checks.

System Calibration Process

Details the automatic calibration process including dark current and wavelength search.

Basic Measurement Procedures

Basic Operation Steps

Outlines the fundamental steps for performing basic measurements.

Performing a Blank Measurement

Instructions for setting the baseline using a blank sample.

Wavelength and Data Management

Setting the Measurement Wavelength

How to input and set the desired wavelength for analysis.

Loading and Deleting Stored Data

Procedures for accessing and managing previously saved data files.

Data Handling and Saving

Loading or Deleting Data/Curves

Instructions for loading existing curves or deleting stored data.

Saving Measurement Data

Steps to save current measurement data or curves.

Data Management and Reporting

Saving Measurement Curves

Details on how to save spectral curves and name them.

Printing Test Results

Instructions for printing measurement reports.

Pre-Measurement Preparation

Printing Test Reports

How to print the results of a completed test.

Preparing for Measurement

Essential steps to prepare the instrument and samples before analysis.

Analyzing Samples with Basic Mode

Basic Mode Operation

Guide to using the basic mode for sample analysis.

Test Mode Selection

Options for selecting different display modes (T%, Abs, Conc/Factor).

Measurement Modes in Basic Operation

Absorbance Measurement Mode

Steps for performing measurements in Absorbance mode.

Transmittance Measurement Mode

Steps for performing measurements in Transmittance (%T) mode.

Concentration/Factor Mode

How to set up and use concentration and factor measurements.

Quantitative Analysis and Reporting

Printing Basic Mode Results

How to print the report for basic mode tests.

Quantitative Test Procedure

Step-by-step guide for performing quantitative analysis.

Quantitative Analysis Setup

Setting Concentration Units

How to select and input units for concentration measurements.

Selecting Correction Methods and Wavelength

Choosing correction methods and setting the analysis wavelength.

Parameter Selection for Analysis

Selecting additional parameters relevant to the quantitative test.

Standard Curve Establishment

Fitting Methods for Curves

Describes different methods available for fitting curves to data points.

Establishing a Standard Curve

Process for creating a standard curve using known samples.

Entering Standard Concentrations

Inputting concentration values for standard samples.

Curve Drawing and Calibration Saving

Drawing the Standard Curve

Visualizing the established standard curve.

Saving Calibration Data

How to save the calibration settings and standard curve.

Performing the Quantitative Test

Steps to execute the quantitative analysis using the established calibration.

Quantitative Test Reporting

Printing Quantitative Test Results

How to print the report for the quantitative analysis.

Wavelength Scan Analysis

WL Scan Operation

Instructions for performing a full wavelength scan of a sample.

Performing Wavelength Scans

Sample Scan Setup

Configuring parameters such as start/end wavelength, step, and speed.

Selecting Scan Test Mode

Choosing the measurement mode (Abs, %T, or E) for the scan.

Wavelength Scan Display Adjustment

Adjusting Scan Graph Scales

Modifying the X and Y axis scales for better visualization of scan data.

Analyzing Scan Data

Searching Scan Data

Methods for searching specific values within the scan results.

Peak-to-Peak Value Search

Locating and identifying peaks and troughs in the scan spectrum.

Scan Data Management

Point-to-Point Scan Search

Searching for specific data points along the scan spectrum.

Saving and Printing Scan Curves

Procedures for saving scan data and printing reports.

Kinetics Analysis

Kinetics Measurement Procedure

Step-by-step instructions for conducting kinetic measurements.

Kinetics Measurement Setup

Setting Time Parameters

Configuring total time, delay time, and time intervals for kinetics.

Selecting Kinetics Test Mode

Choosing between Absorbance or Transmittance modes for kinetics.

Kinetics Data Processing and Analysis

Processing Kinetics Data

Calculating results using begin time, end time, and factor.

Changing Kinetics Graph Scale

Adjusting the scale of the kinetics graph.

Searching Kinetics Data

Locating specific data points on the time-based kinetics graph.

Kinetics Data Management

Saving Kinetics Curves

How to save the recorded kinetics data.

Printing Kinetics Test Reports

Generating and printing reports for kinetics measurements.

DNA/Protein Analysis Procedure

Guide to performing DNA and Protein concentration measurements.

DNA/Protein Measurement Setup

Customizing Analysis Algorithms

Setting custom values for analysis algorithm parameters (f1-f4).

Selecting DNA/Protein Test Mode

Choosing between different modes for DNA/Protein analysis.

DNA/Protein Measurement Execution

Setting Concentration Units

Selecting the unit for displaying concentration results.

Testing DNA/Protein Samples

Steps for loading samples and obtaining test results.

Multi Wavelength Analysis

Multi Wavelength Test Procedure

Instructions for performing measurements at multiple wavelengths.

Multi Wavelength Measurement Setup

Setting Measurement Wavelengths

Inputting a list of wavelengths for multi-wavelength analysis.

Selecting Multi Wavelength Mode

Choosing the measurement mode (Abs or %T) for multi-wavelength tests.

Multi Wavelength Data Management

Saving Multi Wavelength Data

How to save the results of multi-wavelength tests.

Printing Multi Wavelength Reports

Generating and printing reports for multi-wavelength analysis.

System Settings and Calibration

Utility Menu Access

Accessing the utility menu for instrument settings and calibration.

Utility Functions - Printer Setup

WL Reset Functionality

Resetting the wavelength to a default value.

Printer Configuration

Setting up the printer, selecting ports, and choosing print modes.

Utility Functions - Lamp Management

Lamp Control and Usage Reset

Managing the Deuterium (D2) and Tungsten (W) lamps, including switching and resetting usage times.

Utility Functions - Clock Settings

Clock and Display Mode Configuration

Setting the instrument's clock and configuring how time/date are displayed.

Utility Functions - Dark Current and Validity

Dark Current Measurement

Procedure for measuring the dark current of the instrument.

Photometric Accuracy Validation

Performing tests to validate photometric accuracy.

Photometric Accuracy Validation Steps

Setting Wavelength for Validation

Inputting the specific wavelength for photometric validation.

Setting Standard Values

Entering the standard values against which the instrument will be tested.

Selecting Photometric Test Mode

Choosing between Absorbance or Transmittance mode for the test.

Photometric Validation Test Execution

Setting Test Tolerance

Defining the acceptable tolerance range for the photometric test.

Blanking and Sample Check

Performing blanking and then checking the sample against standards.

Wavelength Accuracy Validation

Photometric Validity Test Results

Display and interpretation of results from photometric validation tests.

WL Validity Test Procedure

Steps for performing wavelength validity tests.

Wavelength Validity Test Setup

Setting Standard Peaks for Validation

Inputting standard peak wavelengths for validation.

Selecting Wavelength Test Mode

Choosing between Absorbance or Transmittance mode for wavelength tests.

Setting Wavelength Tolerance

Defining the acceptable tolerance for wavelength measurements.

Wavelength Validity Test Execution

Blanking and Sample Check for WL Validity

Performing blanking and checking samples for wavelength validity.

Connecting to PC

Instructions for establishing a connection between the instrument and a PC.

System Management and Defined Tests

Beeper On/Off Control

Enabling or disabling the audible beeper.

Managing Saved Files

Procedures for deleting all saved measurement files.

Restoring Default Parameters

Resetting the instrument to its factory default settings.

Defined Test Operations

Navigating to the predefined test options.

Configuring Defined Tests

Setting Defined Test Method

Selecting the specific method for a defined test.

Selecting Defined Test Mode

Choosing the measurement mode (Abs or %T) for the defined test.

Executing Defined Tests - References

Placing Reference Samples

Instructions for positioning reference samples in the instrument.

Executing Defined Tests - Samples

Placing Sample Samples

Instructions for positioning sample samples in the instrument.

Appendix A: DNA/Protein Test Algorithm

DNA Measurement Methods

Details on algorithms for DNA concentration and purity measurements.

DNA/Protein Absorbance Ratio

Method for calculating the absorbance ratio for DNA/Protein analysis.

Appendix B: Lamp Replacement

Deuterium Lamp Replacement

Step-by-step guide for replacing the Deuterium lamp.

Deuterium Lamp Replacement Steps

Removing Instrument Covers

Detailed instructions on removing protective covers for lamp access.

Disconnecting Lamp Connectors

How to disconnect electrical connectors related to the lamp assembly.

Deuterium Lamp Replacement - Assembly

Replacing the Lamp Unit

Installing the new Deuterium lamp assembly.

Reconnecting Lamp Connectors

Re-establishing electrical connections for the new lamp.

Deuterium Lamp Alignment and Reset

Refitting Protection Covers

Reassembling protective metal and main covers.

Checking Lamp Alignment

Verifying and adjusting the alignment of the lamp on the slit.

Resetting Lamp Usage Time

Resetting the usage timer for the Deuterium lamp.

Tungsten-Halogen Lamp Replacement

Tungsten-Halogen Lamp Replacement Steps

Guide for replacing the Tungsten-Halogen lamp.

Tungsten-Halogen Lamp Alignment and Reset

Handling Lamps Safely

Precautions for handling hot lamps and avoiding contamination.

Refitting Lamp Components

Reassembling protective covers and screws after lamp replacement.

Resetting Tungsten Lamp Usage Time

Resetting the usage timer for the Tungsten-Halogen lamp.

Appendix C: Interference Correction Techniques

Overview of Correction Techniques

Discusses methods to reduce or eliminate interference errors in measurements.

Isoabsorbance Correction Method

Explains how to correct for interference using a reference wavelength.

Interference Correction Methods

Three-Point Correction Method

Details the three-point correction technique using two reference wavelengths.

Advanced Interference Correction

Linear Interpolation for Background Correction

Describes using linear interpolation to estimate and correct background absorbance.

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