Waters 2489 - Page 2

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June 11, 2015, 715004752 Rev. A

Page ii

Main Page

Trademarks3
Customer Comments3
Contacting Waters4
Safety Considerations4
Safety Hazard Symbol Notice4
Considerations Specific to the 2489 Uv/Visible Detector4
FCC Radiation Emissions Notice5
Electrical Power Safety Notice5
Equipment Misuse Notice5
Applicable Symbols5
Intended Use of the 2489 Uv/Visible Detector6
Calibrating6
Quality Control6
EMC Considerations7
Canada Spectrum Management Emissions Notice7
ISM Classification: ISM Group 1 Class B7
EC Authorized Representative7
Table of Contents9
Theory and Principles of Operation15
Detector Description15
Principles of Operation17
Detector Optics17
Wavelength Verification and Test21
Operational Modes22
Single-Wavelength Mode22
Dual-Wavelength Mode24
Spectrum Scanning25
Cuvette Operations26
Ratioplot26
Maxplot26
Thermal-Wander Management26
Installing the Detector27
Before You Begin27
Unpacking and Inspecting27
Unpacking the Detector27
Inspecting the Detector28
Selecting a Laboratory Site28
Stacking System Modules29
Connecting to the Electricity Source30
Connecting Signal Cables31
Connecting I/O Cables32
Connecting Signal Cables from the Rear Panel and Ethernet Connectors to the Detector33
Making Ethernet Connections33
Starting a Method35
Turning the Detector Lamp on or off36
Connecting the Detector to the 2695 Separations Module36
Connecting the Detector to a Chromatography Data Workstation Using an E-SAT/IN Module38
Connecting the Detector to a 745/745B/746 Data Module41
Connecting the Detector to a Fraction Collector42
Plumbing the Detector43
Connecting Columns in HPLC Systems44
Connecting Columns in ACQUITY Arc Systems44
Assembling the Fittings (HPLC Only)45
Making Tubing Connections in HPLC Systems45
Making Tubing Connections in ACQUITY Arc Systems45
Prepare the Detector47
Initializing the Detector47
Diagnostic Test Failure48
Idle Mode48
Using the Operator Interface49
Using the Display49
Using the Keypad51
Navigating the User Interface55
Primary and Secondary Functions57
Operating Other Detector Functions63
Operating the Detector66
Verifying that the Detector Operates Properly67
Wavelength Calibration69
Operating the Detector in Single-Wavelength Mode69
Operating the Detector in Dual-Wavelength Mode70
Scanning Spectra78
Before You Begin78
Scanning New Spectra80
Storing a Spectrum86
Reviewing a Stored Spectrum87
Subtracting a Spectrum87
Replaying a Spectrum88
Scanning Using the Cuvette88
Scanning Using a Flow Cell and a Syringe90
Conserving Lamp Life90
Shutting down the Detector92
Maintaining the Detector93
Contacting Waters Technical Service93
Maintenance Considerations93
Safety and Handling94
Spare Parts94
Proper Operating Procedures94
Routine Maintenance94
Maintaining the Flow Cell95
Flushing the Flow Cell95
Removing and Cleaning the Flow Cell97
Disassembling and Reassembling the Flow Cell98
Replacing the Lamp103
Lamp Characteristics104
Lamp Energy and Performance104
Removing the Lamp105
Installing the New Lamp107
Recording the New Lamp Serial Number109
Setting the Lamp Threshold110
Replacing Fuses111
Error Messages, Diagnostic Tests, and Troubleshooting113
Error Messages113
Startup Error Messages113
User-Selected Diagnostic Tests113
Overview113
Using the Diagnostic Tests115
Service Diagnostic Tests120
Troubleshooting121
Diagnostic Tests121
Power Surges121
Hardware Troubleshooting121
Lamp Troubleshooting123
A Safety Advisories125
Warning Symbols125
Specific Warnings126
Notices127
Bottles Prohibited Symbol128
Required Protection128
Warnings that Apply to All Waters Instruments and Devices128
Warnings that Address the Replacing of Fuses131
Electrical and Handling Symbols133
Electrical Symbols133
Handling Symbols134
B Specifications137
Physical Specifications137
Environmental Specifications137
Electrical Specifications138
Performance Specifications139
Optical Component Specifications139
Flow Cell Specifications140
C Solvent Considerations141
Introduction141
Preventing Contamination141
Clean Solvents141
Solvent Quality141
Preparation Checklist141
Water142
Using Buffers142
Tetrahydrofuran142
Solvent Miscibility142
How to Use Miscibility Numbers143
Buffered Solvents144
Head Height144
Solvent Viscosity144
Mobile Phase Solvent Degassing145
Gas Solubility145
Solvent Degassing Methods146
Sparging146
Vacuum Degassing146
Solvent Degassing Considerations146
Wavelength Selection147
UV Cutoffs for Common Solvents147
Mixed Mobile Phases148
Wavelength Selection for Chromophore Detection149

Waters 2489 - Page 2

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