Waters 2998 User Manual (English)

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Questions and Answers:

Gregory BrooksJul 25, 2025

Why is there a change in the reference spectrum in Waters 2998 Security Sensors?

S
Stephanie MaloneJul 25, 2025
A change in the reference spectrum may occur in Waters Security Sensors if the mobile phase contains gas or is contaminated. To resolve this, prepare a fresh mobile phase and degas it thoroughly. Another cause might be air bubbles trapped in the flow cell. In this case, reseat and check the alignment of the flow cell. You can also flush the flow cell or apply slight backpressure of 207 to 345 kPa (2 to 3 bar, 30 to 50 psi) on the detector waste outlet by connecting a 1- to 2-foot (30- to 60-cm) length of 0.009-inch (0.23-mm) ID tubing to the detector waste outlet.

Kathleen CruzAug 10, 2025

What to do if both LEDs are unlit on Waters 2998?

G
gonzalezmartinAug 11, 2025
If both LEDs are unlit on your Waters Security Sensors, it could be due to a couple of reasons. First, check if there is electrical power by inspecting the line cord connections and testing the electrical outlet. Second, the fuse may be open or defective, in which case you should replace it.

Category	Photodiode Array Detector
Wavelength Range	190-800 nm
Light Source	Deuterium and Tungsten lamps
Data Acquisition Rate	80 Hz
Pixel Resolution	1.2 nm per pixel
Operating Temperature	15-35 °C
Power Requirements	100 to 240 VAC, 50 to 60 Hz

2998 PDA Detector Optics Principles

Detector Optics

Explains the UV/Vis spectrophotometer, its components, and light path.

Flow Cell Operating Principles

Describes the functionality of the TaperSlit™ flow cell for RI immunity.

Resolving Spectral Data

Discusses how slit width, grating, and photodiode spacing affect spectral resolution.

Measuring Light at the Photodiode Array

Explains how photodiodes measure light intensity for absorbance calculation.

Computing Absorbance Data Points

Details the calculation of absorbance based on detector readings.

Setting Up the Detector

Unpacking and Inspecting

Guides on checking detector contents, condition, and saving packaging.

Laboratory Site Selection

Provides recommendations for choosing a suitable location for reliable operation.

Detector Dimensions

Provides the physical dimensions of the detector unit for installation planning.

Connecting to the Electricity Source

Details how to connect the detector to a grounded power supply.

Making Signal Connections

Explains how to connect the detector to external devices like computers and injectors.

Plumbing the Detector

Instructions for connecting inlet/outlet tubing for fluid flow.

Starting Up and Shutting Down the Detector

Procedures for powering the detector on, warm-up, and powering off.

Using a Cuvette

Describes the procedure for inserting and using the optional cuvette.

Maintaining the Detector

Contacting Waters Technical Service

Information on how to contact technical support with problem details.

Routine Maintenance

Outlines minimal maintenance tasks for optimal performance and longevity.

Maintaining the Flow Cell

Procedures for flushing, removing, and installing the flow cell.

Replacing the Lamp

Steps for safely replacing the detector's source lamp.

Replacing the Fuses

Instructions for replacing the detector's fuses if they fail.

Diagnostic Tests and Troubleshooting

Diagnostic Tests

Explains internal diagnostic tests run at startup and during operation.

Verifying Detector Calibration

Procedure to verify detector calibration after flow cell replacement.

Performing the Erbium Calibration

Details how to perform a manual erbium calibration for wavelength accuracy.

General Troubleshooting

Provides common troubleshooting tips for detector issues and system components.

Spectral Contrast Theory

Comparing Absorbance Spectra

Explains how absorbance spectra characterize compounds and their differences.

Representing Spectra as Vectors

Describes how spectra are converted into vectors for quantitative analysis.

Spectral Contrast Angles

Defines spectral contrast angles and their meaning in comparing spectra shapes.

Undesirable Effects

Discusses factors like noise, error, and solvent changes affecting spectral shape.

Safety Advisories

Warning Symbols

Explains warning symbols used to indicate risks of death, injury, or adverse reactions.

Warnings that Apply to All Waters Instruments

General warnings applicable to all Waters instruments, including modifications.

Electrical and Handling Symbols

Explains electrical symbols and handling symbols for safe operation and transport.

Specifications

Physical Specifications

Details the physical dimensions and weight of the detector.

Environmental Specifications

Outlines operating and storage environmental conditions.

Electrical Specifications

Lists electrical attributes like protection class, voltage range, and fuse type.

Performance Specifications

Lists key performance metrics like wavelength range, resolution, noise, and linearity.

Solvent Considerations

Introduction

General advice on using clean solvents for optimal results and minimal maintenance.

Solvent Miscibility

Provides tables and guidance on predicting solvent miscibility for mobile phase preparation.

Mobile Phase Solvent Degassing

Explains the importance of degassing mobile phases for stable baselines and sensitivity.

Solvent Degassing Methods

Describes common techniques like sparging and vacuum degassing.

Wavelength Selection

Guidance on selecting appropriate wavelengths for detection based on solvent UV cutoffs.

Waters 2998 User Manual

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