Waters 2998 - Using a Cuvette

2-22 Setting Up the Detector
2998 PDA Detector cuvette holder with the cuvette inserted
Restriction: Because the measurement is actually a composite of both the
contents of the cuvette and the flow cell, you need to perform cuvette
measurements under identical flow cell conditions. If you store spectra and
acquire new spectra for subtraction, you need to be aware of differences, if
any, in flow cell conditions.
Ideally, you should perform both the zero and sample measurements using the
cuvette when the HPLC instruments are in the idle or static state and
operating under identical flow cell conditions.
Before you begin
Recommendation: To ensure accurate results, use a 10-mm path length
quartz cuvette and matched pairs (from the same manufacturing lot) of quartz
cuvettes for your zero and sample measurements.
Before beginning a measurement using the cuvette:
1. Fill the flow cell by flushing with 10 mL of the same mobile phase that
you are going to use with the cuvette measurement.
2. To ensure a clear and clean cuvette, wipe the clear portion of the cuvette
with low-lint, nonabrasive tissue wipes.
Caution: Handle the cuvette gently and on the frosted sides only.
Fingerprints on the clear quartz interfere with the light path and
compromise the integrity of cuvette measuring operations.
Frosted sides of cuvette
facing up and down