Undesirable effects 5-7
Normalized absorbance spectra of a compound at two concentrations
Undesirable effects
Shape differences between absorbance spectra can be caused by one or more of
the following undesirable effects:
• Detector noise
• Photometric error caused by high sample concentration
• Variation in solvent composition
These sources of spectral variation can cause chemically pure,
baseline-resolved peaks to exhibit a small level of spectral inhomogeneity. You
can assess the significance of spectral inhomogeneity by comparing a spectral
contrast angle to a threshold angle (see page 5-8).
Normalized spectra of a compound at different
concentrations
Spectral contrast angle: 3.4°
Wavelength (nm)
Region of little or no
analyte absorption
Normalized absorbance
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