2-16 Setting Up the Detector
Plumbing the detector
See also: “Minimum tubing bend radius recommendations” on page C-6.
Required Materials
• 5/16-inch open-end wrench
• 0.009-inch (0.23 mm) ID stainless steel tubing (included in the Startup
Kit)
• Stainless steel tubing cutter or scribing file
• Pliers, plastic-, or cloth-covered
• Compression screw assemblies (3)
To make plumbing connections to the detector:
1. Measure the length of tubing needed to connect the column outlet to the
detector inlet.
Tip: Keep the length of this tubing as short as possible to prevent band
broadening.
2. Measure the length of tubing needed to connect the detector outlet to a
waste collection bottle.
Tip: Ensure the length of this tubing is at least 30 to 60 cm (1 to 2 feet)
to prevent air bubbles from forming in the flow cell.
3. Cut the two lengths of tubing as follows:
Warning:
• To avoid chemical hazards, always observe Good Laboratory
Practices when operating your system. Refer to the Material Safety
Data Sheets shipped with solvents for handling information.
• Using incompatible solvents can cause severe damage to the
instrument and injury to the operator.
Caution: To prevent contamination, wear powder-free, nonlatex gloves
when plumbing the detector.
Caution: To avoid damaging the flow cell, never cut the 1/32-inch
stainless steel flow cell tubing.
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