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Waters 2489 User Manual

Waters 2489
234 pages
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Waters 2489
UV/Visible Detector
Operators Guide
71500142102 / Revision A
Copyright © Waters Corporation 2007.
All rights reserved.

Table of Contents

Other manuals for Waters 2489

Questions and Answers:

Waters 2489 Specifications

General IconGeneral
TypeUV/Vis Detector
Wavelength Range190-700 nm
Light SourceDeuterium lamp
Drift<1 x 10^-4 AU/hour
Flow Cell Volume10 μL
Power Requirements100-240 VAC, 50/60 Hz
Bandwidth8 nm

Summary

1 Theory and Principles of Operation

Detector Description

Overview of the Waters 2489 UV/Visible Detector, its applications, and configuration.

Principles of Operation

Explains the optical and electronic design and theory of operation for effective detector use.

Detector Optics

Describes the components of the Waters 2489 UV/Visible Detector optics assembly.

Waters TaperSlit Flow Cell

Explains the TaperSlit Flow Cell's design for improved baseline stability and light throughput.

Operational Modes

Covers single and dual wavelength modes, spectrum scanning, and plot functions.

Single Wavelength Mode

Details the default operational mode for monitoring a single wavelength with primary parameters.

Dual Wavelength Mode

Explains monitoring two wavelengths, sampling frequency, and conditions for filter engagement.

Spectrum Scanning

Details using the detector as a spectrophotometer to acquire and store spectra from flow cell or cuvette.

2 Installing the Detector

Preparing for Installation

Outlines major steps in installing the detector, from startup procedure to final connections.

Site Selection and Power Requirements

Details installation site requirements for temperature, humidity, space, and power.

Power Requirements

Specifies the detector's electrical power needs, including voltage, frequency, and fuse type.

Unpacking and Inspecting

Guides the user on unpacking the detector and checking its contents and condition.

Making Fluid Line Connections

Provides instructions for completing fluid line connections, including column and drip management.

Making Electrical Power Connections

Explains how to connect the detector to the AC power supply.

Making Signal Connections

Guides on making signal connections considering operating mode and HPLC system components.

I/O Signals for the Detector

Describes the signals available on the I/O connectors, including Inject Start, Lamp On/Off, and Analog outputs.

Making Ethernet Connections with Waters Data Systems

Explains connecting via Ethernet to Waters data systems like Empower or MassLynx.

Connecting to Other Devices

Guides on connecting the detector to various HPLC system devices like e-SAT/IN, chart recorders, and pumps.

Connecting the Detector to Empower using an e-SAT/IN Module

Explains connections for acquiring data and controlling the detector with Empower via e-SAT/IN.

3 Preparing the Detector

Initializing the Detector

Covers powering on the detector and the startup diagnostic tests.

Using the Operator Interface

Introduces the detector's operator interface, including the display and keypad.

Detector Absorbance Screen

Details the main screen showing absorbance, wavelength, and sensitivity settings.

Using the Keypad

Provides an overview of the detector's 24-key keypad and its functions.

Detector Keypad Description

Explains the functions of the primary and secondary keys on the detector keypad.

Navigating the User Interface

Guides on navigating editable fields, choice lists, and screens using the detector's interface.

Setting up a Run

Details programming parameters like wavelength, sensitivity, and time constant before starting a run.

Primary and Secondary Functions

Lists functions accessible from the absorbance screen or via the Next key, detailing detector functions.

Detector Functions

Explains functions like Wavelength, AUFS, Analog out, Filter time constant, Voltage offset, and Chart polarity.

Operating Other Detector Functions

Covers configuring the detector, including event inputs and pulse periods.

Operating the Detector

Covers operating the detector in single or dual wavelength modes and stand-alone or remote control.

Verifying the Detector

Outlines procedures for verifying proper detector operation after installation.

Wavelength Calibration

Describes how to manually calibrate the detector from the keypad at any time.

Programming Timed Events, Threshold Events, and Methods

Covers storing and retrieving up to five methods, including timed and threshold events.

Storing a Method

Guides on storing current parameters, timed events, and threshold events as a method.

Scanning Spectra

Covers performing zero scans and sample scans to produce absorbance spectra.

4 Maintaining the Detector

Contacting Waters Technical Service

Provides contact information for Waters Technical Service for HPLC equipment issues.

Maintenance Considerations

Covers safety precautions and spare parts recommendations for detector maintenance.

Safety Precautions

Lists critical safety considerations to keep in mind during maintenance procedures.

Maintaining the Flow Cell

Explains the importance of flow cell cleanliness and the procedures for its maintenance.

Inspecting, Cleaning, and Replacing Damaged Flow Cell Components

Covers inspecting, cleaning, and replacing parts like lenses, windows, and gaskets for the flow cell.

Replacing the Flow Cell

Explains when and how to replace the detector's flow cell assembly.

Replacing the Lamp

Guides on replacing the detector's deuterium lamp, including when to replace it and lamp characteristics.

When to Replace the Lamp

Provides guidelines on when a lamp replacement is necessary based on performance or ignition issues.

Removing the Lamp

Details the procedure for safely removing the old lamp, including cooling and precautions.

Installing the New Lamp

Guides on installing a new lamp, including unpacking, inspection, and positioning.

Replacing Fuses

Guides on locating and replacing the detector's fuses, including type and rating.

5 Error Messages, Diagnostic Tests, and Troubleshooting

Error Messages

Describes powerup, calibration, and operating error messages and recommended corrective actions.

Error Messages Preventing Operation

Details fatal errors that halt detector operation and require immediate attention.

User-Selected Diagnostic Tests

Covers operating diagnostic tests for troubleshooting and verifying detector electronics and optics.

To Perform User-Selectable Diagnostic Tests

Guides on accessing and running diagnostic tests from the detector's front panel.

Using the Diagnostic Tests

Explains how to access and use user-selectable and service diagnostic tests.

Using the Change Lamp Diagnostic Test

Details the diagnostic test for changing the lamp, including serial number and installation date entry.

Troubleshooting

Provides causes of errors and recommended troubleshooting actions, including contacting Waters.

Hardware Troubleshooting

Offers general hardware troubleshooting for common detector issues like inoperative status or display problems.

A Safety Advisories

Warning Symbols

Alerts to risks like death, injury, or adverse reactions associated with instrument use or misuse.

Task-Specific Hazard Warnings

Highlights specific risks like burn injuries, electric shocks, and UV radiation exposures.

B Detector Specifications

Operational Specifications

Lists key operational parameters like wavelength range, bandwidth, noise levels, linearity, and drift.

Optical Component Specifications

Details specifications for the lamp source, photodiodes, second order filter, and flow cell.

Optical Specifications

Provides specifications for the monochromator type, grating, optical bandwidth, and lamp power.

C Spare Parts

D Solvent Considerations

Solvent Miscibility

Provides a table and guidelines for determining solvent miscibility before changing solvents.

Mobile Phase Solvent Degassing

Highlights the importance of degassed solvents for stable baselines, sensitivity, and reproducibility.

Solvent Degassing Methods

Covers techniques like Sparging with helium and Vacuum degassing for solvent degassing.

Wavelength Selection

Covers UV cutoff ranges for common solvents, mixed mobile phases, and chromophores.

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