Waters 2489 - Recording Sample and Reference Beam Energies

3-28 Preparing the Detector
reference beam energies” on page 3-28 before pumping any mobile phase or
solvent through the flow cell.
To pump solvent through the unit before initial use:
1. Connect the detector to a data system or chart recorder. Refer to
Chapter 2 for complete information on connecting the detector to
external devices.
2. Power-on the detector. The front panel displays a series of initialization
messages for about 5 minutes. Refer to “Initializing the detector” on
page 3-2.
3. When initialization is complete, the detector displays the absorbance
screen. For an explanation of the detector keypad, see “Using the
keypad” on page 3-6.
4. Allow the detector to warm up for at least 30 minutes before operating.
Tip: If the startup verification diagnostic tests fail, note the error
message to determine the corrective action. Refer to Chapter 5.
Recording sample and reference beam energies
To determine baseline values on your detector for future reference and to
monitor lamp aging (decreased lamp output energy), you need to record the
baseline sample and reference beam energies to compare against future
readings. Use these baseline values to troubleshoot the detector to determine
whether
• the solvent is contaminated.
• the flow cell is contaminated.
• the lamp needs to be replaced.
• there is an air bubble in flow cell.
To record the sample and reference beam energies:
1. On the absorbance screen, use the arrow keys to highlight the λ field.
2. Enter 230 into the λ field, and then press Enter.