3-62 Preparing the Detector
Rules:
• To prevent invalid subsequent chromatographic results, remove the
cuvette from the detector and replace the empty holder after
running your cuvette scans.
• To maintain optimum system performance, replace the front left
panel cover before resuming normal operation of the detector.
6. Insert a reference cuvette containing the mobile phase standard and run
a zero scan.
7. Replace the reference cuvette with a cuvette containing your analyte
dissolved in mobile phase solvent and run a sample scan.
8. Use the storage, review, subtract and review, and replay functions to
analyze the data obtained.
Scanning using a flow cell and a syringe
If you do not have a cuvette, you can scan using a manually filled flow cell.
Requirement: Before you scan using a flow cell, make sure there is no cuvette
in the cuvette holder.
To run a spectrum using a flow cell:
1. With a syringe, fill the flow cell with the mobile phase or solvent in
which your sample is dissolved.
2. Run a zero scan following the procedure “Scanning new spectra” on
page 3-48.
3. With a syringe, fill the flow cell with the analyte, then run a sample scan
following the procedure in “Scanning new spectra” on page 3-48.
Use the storage, review, subtract and review, and replay functions of the
detector to compare the scanned data.
Conserving lamp life
You can conserve the lamp without powering-off the detector by leaving the
instrument on and turning off only the deuterium lamp.
Tip: If the detector is operating under remote control, you can program the
controller to turn the lamp off and on without using the detector front panel.
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