Waters 2489 - Electronic Absorption Bands of Representative Chromophores*

D-12 Solvent Considerations
particular analysis. Because of the diversity possible within a given sample,
scanning over a range of wavelengths may be necessary to determine the best
wavelength for a particular analysis.
Electronic absorption bands of representative chromophores*
Chromophore
Chemical
Configuration
λ
max
(nm)
∈
max
(L/m/cm)
λ
max
(nm)
∈
max
(L/m/cm)
Ether —O— 185 1000
Thioether —S— 194 4600 215 1600
Amine —NH
2
195 2800
Thiol —SH 195 1400
Disulfide —S—S— 194 5500 255 400
Bromide —Br 208 300
Iodide —I 260 400
Nitrile —C≡N 160 —
Acetylide —C≡C— 175-180 6000
Sulfone —SO
2
— 180 —
Oxime —NOH 190 5000
Azido >C=N— 190 5000
Ethylene —C=C— 190 8000
Ketone >C=O 195 1000 270-285 18-30
Thioketone >C=S 205 strong
Esters —COOR 205 50
Aldehyde —CHO 210 strong 280-300 11-18
Carboxyl —COOH 200-210 50-70
Sulfoxide >S—O 210 1500
Nitro —NO
2
210 strong
Nitrile —ONO 220-230 1000-2000 300-400 10
Azo —N=N— 285-400 3-25
Nitroso —N=O 302 100