Waters 2489 - Page 219

Wavelength selection D-13
*Willard, H. H. and others. Instrumental Methods of Analysis, 6th ed. Litton Educational Publishing,
Inc., 1981. Reprinted by permission of Wadsworth Publishing Co., Belmont, California, 94002.
Nitrate —ONO
2
270
(shoulde
r)
12
Allene —(C=C)
2
—
(acyclic)
210-230 21,000
Allene —(C=C)
3
— 260 35,000
Allene —(C=C)
4
— 300 52,000
Allene —(C=C)
5
— 330 118,000
Allene —(C=C)
2
—
(alicyclic)
230-260 3000-8000
Ethylenic/
Acetylenic
C=C—C≡C 219 6,500
Ethylenic/
Amido
C=C—C=N 220 23,000
Ethylenic/
Carbonyl
C=C—C=O 210-250 10,000-
20,000
Ethylenic/
Nitro
C=C—NO
2
229 9,500
Electronic absorption bands of representative chromophores*
Chromophore
Chemical
Configuration
λ
max
(nm)
∈
max
(L/m/cm)
λ
max
(nm)
∈
max
(L/m/cm)