5-1
5 Spectral Contrast Theory
The spectral contrast algorithm compares the UV/Vis absorbance
spectra of samples the detector collects. This chapter describes the
theory on which the algorithm is based, explaining how it exploits
differences in the shapes of the absorbance spectra. It also explains how
spectral contrast represents those spectra as vectors, determining
whether differences among them arise from the presence of multiple
compounds in the same peaks (coelution) or from nonideal conditions
like noise, photometric error, or solvent effects.
Contents:
Topic Page
Comparing absorbance spectra 5-2
Representing spectra as vectors 5-2
Spectral contrast angles 5-4
Undesirable effects 5-7
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