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Waters nanoACQUITY UPLC User Manual
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nanoACQUITY UPLC
System
Qu
ick S
t
art Gu
i
de
7150009750
3/Re
vision D
Copyrigh
t
© W
aters Corpo
ration 2006.
All ri
ght
s reserved
.
CORPORATION
2
Table of Contents
Default Chapter
4
Operating this Device
4
Observing Safety Precautions
11
Using Waters Equipment
12
Safety and Electromagnetic Equipment Compatibility
13
United States - FCC Rules
13
United States - Safety Requirements
13
Canada - Spectrum Management
14
Europe - Safety and Electromagnetic Compatibility
14
Australia - Emissions Requirements
14
Nanoacquity UPLC System Information
15
Intended Use
15
Biological Hazard
15
Quality Control
15
Table of Contents
17
1 System Overview
21
Instruments and Components
21
Nanoacquity Operating Modes
23
Direct Injection
24
Single-Pump Trapping
24
Two-Pump Trapping
25
Two-Dimensional Liquid Chromatography (2D-LC)
25
Nanoacquity Binary Solvent Manager
26
How the Binary Solvent Manager Works
26
Flow Control Modules
26
Sample Manager
27
Sample Consumption Guidelines
28
Auxiliary Solvent Manager
27
Flow Control Module
27
Heating and Trapping Module
29
TUV Optical Detector
30
Mass Detectors
30
Q-Tof Premier
30
LCT Premier
31
Masslynx Data System
31
Nanoacquity UPLC Console
31
2 Preparing System Hardware
33
Powering-On the System
33
Monitoring Startup Tests
34
Monitoring the Leds of System Instruments
34
Power LED
34
Status Leds
35
Preparing the Auxiliary Solvent Manager
36
Priming the Seal Wash
37
Preparing the Binary Solvent Manager
40
Priming the Seal Wash
40
Priming the Binary Solvent Manager
42
Preparing the Sample Manager
44
Selecting Weak and Strong Wash Solvents
44
Priming the Sample Manager
46
Washing the Sample Manager Needle
47
Characterizing the Needle Seal
49
Characterizing the Needle and Sample Loop Volumes
50
Loading Sample Plates in the Sample Manager
51
Preparing the Detector
53
Preparing the TUV Detector
53
Preparing the Mass Spectrometer
54
Conditioning the Column
55
Shutting down the System
56
Between Analyses
56
Overnight or Weekends
56
More than 72 Hours (Long-Term)
57
3 Configuring System Software
59
Starting the Nanoacquity UPLC Console from Masslynx
63
Configuring Events
64
4 Verifying System Operation
65
Required Materials
65
Preparing the Mobile Phases
66
Preparing the Sample
67
Preparing the System
68
Creating the Test Methods
70
Creating the Instrument Method
70
Performing the Test
74
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Waters nanoACQUITY UPLC Specifications
General
Brand
Waters
Model
nanoACQUITY UPLC
Category
Laboratory Equipment
Language
English
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