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Waters ACQUITY UPLC M-Class User Manual

Waters ACQUITY UPLC M-Class
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ACQUITY UPLC M-Class
System Guide
715003588
Revision C
Copyright © Waters Corporation 2019
All rights reserved

Table of Contents

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Waters ACQUITY UPLC M-Class Specifications

General IconGeneral
System TypeUltra Performance Liquid Chromatography (UPLC)
Maximum Pressure15, 000 psi (1034 bar)
Power Requirements100-240 VAC, 50/60 Hz
Flow Rate Range0.001 to 2.0 mL/min
Column Temperature Range4°C to 90°C
Sample Capacity384-well
Detector TypeFLR
Injection Volume0.1 µL
Detection Wavelength Range190 to 800 nm (depending on detector)
DimensionsVaries depending on configuration
WeightVaries depending on configuration

Summary

1 ACQUITY UPLC M-Class system

1.1 Configurations of system modules

Examples of system stack configurations based on core modules and module types.

1.2 System components

Describes the system components and their functions based on installed modules.

1.2.2 Auxiliary Solvent Manager

Details the Auxiliary Solvent Manager (ASM) pump, its capabilities, and configuration.

1.2.4 µSample Manager - Fixed Loop

Explains the µSample Manager - Fixed Loop (µSM-FL) for sample injections and thermal control.

2 Performance optimization

2.1 Best practices to improve performance

Recommends practices for achieving more reproducible chromatography.

2.2 General guidelines for a microscale system

Provides guidelines for microscale UPLC system performance.

2.5 Contamination

Details how to minimize contamination of sample preparation tools and bottles.

2.6 Preventing leaks

Explains how to prevent leaks by properly tightening system fittings.

3 Preparation and operation

3.1 Preparing the system

Outlines steps for preparing the system for operation, including powering on hardware and software.

3.1.2 Configuring the system software

Details how to configure system software, including MassLynx and driver packs.

3.2 Starting the instrument console

Guides on launching and using the instrument console software.

3.3 Instrument control panels

Explains how to monitor and control system modules via control panels.

4 Maintaining the system

4.1 Contacting Waters Technical Service

Provides contact information and required details for Waters Technical Service.

4.2 Maintenance procedure and frequency

Lists periodic maintenance schedules for optimal system performance.

4.3 Troubleshooting

Guides on systematically troubleshooting system malfunctions.

4.5 Spare parts

Lists where to find information on Waters Quality Parts and compatible accessories.

A Safety advisories

A.1 Warning symbols

Explains warning symbols that alert to risks like death, injury, or adverse reactions.

A.4 Required protection

Highlights the requirement for personal protective equipment like eye protection and gloves.

A.5 Burst warning

Warns about bursting nonmetallic tubing under pressure and precautions.

A.7 Warnings that apply to all Waters instruments and devices

Lists general warnings applicable to all Waters instruments and devices.

B Specifications

B.1 Features and performance specifications

Lists performance specifications for the ACQUITY UPLC M-Class system and components.

B.2 Instrument control

Lists instrument control specifications for the ACQUITY UPLC M-Class system.

B.5 Physical specifications

Lists physical specifications for individual ACQUITY UPLC M-Class system components.

C External connections

C.1 System waste routing

Shows the process waste flow for a system stack on a micro cart.

C.2 External wiring connections

Illustrates example process waste management and external wiring connections.

C.4 Connecting to the electricity source

Explains the requirement for a separate, grounded power source for each module.

D Solvent considerations

D.1 Solvent guidelines

Provides general information and warnings regarding solvent use.

D.2 Solvent recommendations

Lists recommended solvents for the ACQUITY UPLC M-Class System.

D.2.4 Operation with neutral or basic mobile phases

Provides recommendations for operating with neutral or basic mobile phases.

D.2.4.5 Cleaning the system

Explains how cleaning the system kills and removes residual bacteria.

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