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Waters Xevo TQ MS User Manual

Waters Xevo TQ MS
248 pages
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Waters Xevo TQ MS
Operators Overview and Maintenance Guide
71500130002 / Revision D
Copyright © Waters Corporation 2013.
All rights reserved

Table of Contents

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Waters Xevo TQ MS Specifications

General IconGeneral
BrandWaters
ModelXevo TQ MS
CategoryLaboratory Equipment
LanguageEnglish

Summary

General Information

Copyright notice

Provides legal notice regarding copyright and reproduction rights.

Trademarks

Lists registered and unregistered trademarks associated with the product and company.

Customer comments

Contacting Waters

Safety considerations

Considerations specific to the Xevo TQ MS

Details specific safety concerns and warnings related to the Xevo TQ MS instrument.

FCC radiation emissions notice

Information regarding compliance with Federal Communications Commission regulations.

Canada spectrum management emissions notice

Information regarding compliance with Canadian spectrum management regulations.

Electrical power safety notice

Guidance on safe placement and operation related to electrical power.

Safety hazard symbol notice

Instructions on consulting documentation for hazard symbols and actions.

Equipment misuse notice

Warning about impaired protection if equipment is used improperly.

Safety advisories

Reference to Appendix A for comprehensive warning and caution advisories.

Operating this instrument

Applicable symbols

Explains various symbols used in the manual and their definitions.

Audience and purpose

Defines the intended audience and the overall purpose of the guide.

Intended use of the Xevo TQ MS

Describes the purpose and application of the Xevo TQ MS instrument.

Calibrating

Provides guidance on the calibration procedures for LC systems and mass spectrometers.

Quality-control

Details recommended procedures for running QC samples and managing data.

ISM classification

ISM Classification: ISM Group 1 Class A

Specifies the instrument's classification according to ISM requirements.

EC authorized representative

1 Waters Xevo TQ MS Overview

Waters Xevo TQ MS

Provides a general overview of the Waters Xevo TQ MS instrument.

Ionization techniques and source probes

Describes the different ionization techniques and source probes compatible with the instrument.

IntelliStart Fluidics system

Details the IntelliStart Fluidics system, its components, and operation.

Ion optics

Explains the operation of the mass spectrometer's ion optics.

MS operating modes

Details the available MS operating modes and their configurations.

MS/MS operating modes

Describes the various MS/MS operating modes, such as MRM and product ion scans.

Leak sensors

Explains the function and operation of the instrument's leak sensors.

Vacuum system

Describes the vacuum system components and their role in instrument operation.

Rear panel

Illustrates and identifies the connectors on the mass spectrometer's rear panel.

2 Preparing the Mass Spectrometer for Operation

Starting the mass spectrometer

Step-by-step guide on how to power on and prepare the mass spectrometer for use.

Verifying the instrument’s state of readiness

Checks to confirm the instrument is in proper operating condition.

Monitoring the mass spectrometer LEDs

Explains the meaning of the operational status indicated by the instrument's LEDs.

Tuning and calibration information

Guidance on performing tuning and calibration procedures before instrument use.

Running the mass spectrometer at high flow rates

Provides parameters for optimizing desolvation and sensitivity at high flow rates.

Preparing the IntelliStart Fluidics system

Instructions for setting up the IntelliStart Fluidics system, including reservoir installation.

Rebooting the mass spectrometer

Steps to restart the mass spectrometer's electronics when software fails or after an upgrade.

Leaving the mass spectrometer ready for operation

Guidelines on when to leave the instrument in Operate mode versus Standby mode.

Emergency shutdown of the mass spectrometer

Procedure for safely shutting down the mass spectrometer in an emergency situation.

3 Changing the Mode of Operation

ESI mode

Instructions for fitting and operating the ESI probe for Electrospray Ionization.

ESCi mode

Guidance on fitting an ESI probe and corona pin for ESCi operation.

APCI mode

Details on installing and operating the APCI probe for Atmospheric Pressure Chemical Ionization.

Combined APPI/APCI source

Information on operating the optional APPI, APCI, or dual-mode APPI/APCI source.

NanoFlow ESI source

Describes the NanoFlow ESI source, its components, and installation.

4 Maintenance Procedures

Maintenance schedule

Lists periodic maintenance tasks to ensure optimum instrument performance.

Spare parts

Information on ordering and replacing spare parts for the instrument.

Troubleshooting with Connections INSIGHT

Guide to using Connections INSIGHT for device management and submitting support requests.

Safety and handling

General safety considerations and handling precautions for performing maintenance.

Preparing the instrument for working on the source

Procedure to prepare the instrument before performing maintenance on the source.

Removing and refitting the source enclosure

Steps for removing and reinstalling the instrument's source enclosure.

Installing and removing the corona pin

Procedure for installing and removing the corona pin for specific operation modes.

Operating the source isolation valve

Instructions for closing and opening the source isolation valve for maintenance.

Removing O-rings and seals

Guidance on using the O-ring removal kit and handling O-rings and seals.

Cleaning the instrument case

Instructions for cleaning the exterior surfaces of the mass spectrometer.

Emptying the nitrogen exhaust trap bottle

Procedure for inspecting and emptying the nitrogen exhaust trap bottle.

Inspecting the Varian roughing pump oil level

Steps for checking and maintaining the oil level in the Varian roughing pump.

Adding oil to the Varian roughing pump

Procedure for adding oil to the Varian roughing pump when the level is low.

Replacing the Varian roughing pump oil and oil mist filter

Annual procedure for changing the roughing pump oil and oil mist filter.

Cleaning the source components

Guidance on cleaning various components within the instrument's source.

Cleaning the sampling cone assembly

Steps for removing, disassembling, and cleaning the sampling cone assembly.

Cleaning the extraction cone

Procedure for cleaning the extraction cone after other components have been cleaned.

Cleaning the ion block assembly

Instructions for cleaning the ion block assembly if signal sensitivity issues persist.

Cleaning the source hexapole assembly

Procedure for cleaning the source hexapole assembly when signal sensitivity is low.

Replacing the ESI probe tip and gasket

Instructions for replacing the ESI probe tip and gasket due to blockage or damage.

Replacing the ESI probe sample capillary

Procedure for replacing the ESI probe's stainless steel sample capillary.

Cleaning the APCI probe tip

Procedure for cleaning the APCI probe tip when buffer buildup or weak signal is detected.

Replacing the APCI probe sample capillary

Instructions for replacing the APCI probe's sample capillary if blocked or damaged.

Cleaning or replacing the corona pin

Guidance on cleaning or replacing the corona pin for APCI and ESCi modes.

Replacing the APCI probe heater

Procedure for replacing the APCI probe heater if it fails to heat.

Replacing the ion block source heater

Procedure for replacing the ion block source heater if it fails to heat.

Replacing the source assembly seals

Instructions for replacing various seals in the source assembly to prevent solvent vapor leakage.

Replacing the air filter

Instructions for annually replacing the instrument's air filter.

APPI/APCI source—changing the UV lamp bulb

Steps for safely changing the UV lamp bulb in the APPI/APCI source.

APPI/APCI source—Cleaning the lamp window

Procedure for cleaning the magnesium fluoride lamp window for optimal photon transmission.

APPI/APCI source—Replacing the APPI lamp drive seals

Instructions for replacing O-rings in the APPI lamp drive assembly annually.

A Safety Advisories

Warning symbols

Explains various warning symbols and their associated risks and precautions.

Caution advisory

Highlights caution advisories related to instrument use that could damage it or compromise sample integrity.

Warnings that apply to all Waters instruments and devices

General warnings applicable to all Waters instruments and devices regarding modifications.

Warnings that address the replacing of fuses

Safety warnings and instructions related to replacing fuses in the instrument.

Electrical and handling symbols

Lists electrical and handling symbols with their associated statements and meanings.

Electrical symbols

Explains common electrical symbols found on instruments and in manuals.

Handling symbols

Defines common handling symbols found on packaging and labels.

B Materials of Construction and Compliant Solvents

Preventing contamination

Reference to a document for preventing contamination in the IntelliStart Fluidics system.

Items exposed to solvent

Table listing instrument components and their material exposed to solvents.

Solvents used to prepare mobile phases

Lists common solvents used for mobile phases in reverse-phase LC/MS.

C External Connections

External wiring and vacuum connections

Diagram and explanation of the mass spectrometer's rear panel connectors.

Connecting the Varian oil-filled roughing pump

Step-by-step guide for connecting the Varian oil-filled roughing pump.

Connecting the Edwards oil-free roughing pump

Instructions for connecting the alternative Edwards oil-free roughing pump.

Connecting to the nitrogen gas supply

Procedure for connecting the instrument to a nitrogen gas supply.

Connecting to the collision cell gas supply

Steps for connecting the argon gas supply to the collision cell.

Connecting the nitrogen exhaust line

Instructions for connecting the nitrogen exhaust line to the trap bottle and exhaust system.

Connecting the liquid waste line

Procedure for connecting the instrument's liquid waste line to a container.

Connecting the workstation

Guidance on setting up and connecting the workstation to the mass spectrometer.

Connecting Ethernet cables

Steps for making Ethernet connections between the workstation and instrument.

Input/output signal connectors

Information on the instrument's rear panel connectors for input/output signals.

Connecting to the electricity source

Instructions for connecting the instrument to a suitable electricity source.

D Plumbing the IntelliStart Fluidics System

Preventing contamination

Reference to a document for preventing contamination in the IntelliStart Fluidics system.

Plumbing schematic

Diagram illustrating the plumbing layout of the IntelliStart Fluidics system.

Pipe specifications

Table detailing the specifications for replacement pipes used in the IntelliStart Fluidics system.

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