Why is there increased system backpressure during injection using Rheodyne injector in Waters HPLC?
Increased system backpressure during injection with a Rheodyne injector in Waters Laboratory Equipment is normal. When the injector is in the Inject position, the sample loop generates additional system backpressure. To decrease the system backpressure, return the injector to the LOAD position after loading the sample.
What to do if the Waters HPLC Laboratory Equipment system controller screen is blank?
If the screen on your Waters Laboratory Equipment system controller is blank, the screen saver feature may be active. Press any function key to display the screen.
What to do if my Waters Laboratory Equipment pump keeps shutting down due to exceeding the high-pressure limit?
If your Waters Laboratory Equipment pump shuts down because the high-pressure limit is exceeded, there are several potential causes. It could be due to a high flow rate during injection valve actuation, causing a pressure spike; in this case, decrease the flow rate during injection. Another cause may be a blockage in the system after the transducer; try removing components between the pump and the end of the system to locate and eliminate the blockage. Finally, the column might be plugged, so try cleaning or replacing it.
Why is my Waters HPLC Laboratory Equipment pump cavitating?
Pump cavitation in your Waters Laboratory Equipment can occur for several reasons. First, check the solvent reservoirs' position; if they are below the solvent proportioning valve, place them on the bench beside the fluid handling unit. Second, inspect tubing connections and fittings for leaks prior to the inlet check valve, as these can cause air aspiration; eliminate any leaks by tightening the fittings or replacing the ferrules. Lastly, the solvent inlet filter might be restricting flow, so clean or replace the filters.
What to do if Waters HPLC pump shuts down because the low-pressure limit is exceeded?
If your Waters Laboratory Equipment pump shuts down because the low-pressure limit has been exceeded, the issue is likely due to leaks, empty reservoirs, or bubbles in the pump head. Eliminate any leaks, fill the reservoirs, and prime the pump to resolve this.
How to fix a grinding or shuddering noise from Waters HPLC Laboratory Equipment pump?
If you notice a grinding or shuddering noise from your Waters Laboratory Equipment pump, and the high-pressure shutdown has not been exceeded, it could be due to a blockage in the system before the transducer. Clean the pump head outlet tubing, outlet check valve, and the tubing leading to the transducer. If the problem persists, it may be a mechanical issue.
Why is there a pulsing flow from the waste line of my Waters HPLC Laboratory Equipment?
A pulsing flow from the waste line in your Waters Laboratory Equipment is often caused by dirty check valve(s). Clean the check valve(s) to resolve this issue.
What to do if Waters HPLC RI detector is leaking internally?
If your Waters Laboratory Equipment RI detector is leaking internally or from the purge port, the cell may be damaged because the maximum flow rate or pressure of the RI cell has been exceeded. Ensure you do not exceed 5 mL/min for 2414, 2410, and 410 Detectors, and do not exceed 100 psi for any Waters RI detector.
Why are peak retention times halved in Waters HPLC Laboratory Equipment?
Halved peak retention times in Waters Laboratory Equipment are caused by an incorrect pump head volume entered on the PUMP CONFIGURATION screen. For PrepLC operation with 1000-µL pump heads, enter 1000 for the pump head volume on the PUMP CONFIGURATION screen.
Why can't I enter a flow rate above 150 mL/min on my Waters HPLC?
If you are unable to enter a flow rate above 150 mL/min on your Waters Laboratory Equipment, it is because an incorrect pump head volume was entered on the PUMP CONFIGURATION screen. For operation with 1000-µL pump heads, enter 1000 for the pump head volume on the PUMP CONFIGURATION screen.
Details the features and configurations of the Waters Prep Series HPLC systems.
Provides initial steps for setting up and operating the system.
Covers unpacking, inspecting components, and selecting installation site.
Details voltage, frequency, and fuse specifications for system operation.
Explains how to connect components like injectors, modules, and fluid handling unit.
Provides guidance for identifying and resolving system problems.
Outlines tests to isolate problem causes and verify functionality.
Explains hazard symbols and their associated risks for safe operation.
Describes caution symbols indicating potential damage or sample compromise.
Lists operational parameters such as solvent number, flow range, and pressure limits.
Details power requirements, consumption, frequency, and fuse types.
Lists commonly used spare parts with part numbers.
Lists spare parts specific to the 3725 injector.
Emphasizes the importance of clean, particle-free solvents for reproducible results.
Provides guidance on solvent miscibility and properties for solvent changes.
General| Brand | Waters |
|---|---|
| Flow Rate Range | 0.001-10 mL/min |
| Pressure Range | Up to 15, 000 psi |
| Detector Types | UV/Vis, PDA, Fluorescence, Refractive Index, ELSD |
| Wavelength Range (UV/Vis) | 190-800 nm |
| Data Management | Empower Software |
| Compliance | 21 CFR Part 11, GMP/GLP |
| Type | High-Performance Liquid Chromatography (HPLC) |
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