D-2 Solvent Considerations
Clean solvent
The use of clean, particle-free solvents is important not only for reproducible
results but also for operation with minimal maintenance. An unclean solvent
can cause baseline noise and drift. Recommended solvent preparation includes
using Milli-Q
®
water and filtering through a 0.45-µm filter.
When operating a system with water, clean the water just before use to
eliminate the presence of bacteria. To clean water, distill it in a glass and pass
it through a Norganic water purification system or prepare water with the
Milli-Q system. Contact your Waters
®
representative for more information.
Inlet solvent filters can be used at low flow rates. To avoid drawing foreign
particles into the pump where they can accumulate and affect pump
operation, keep the solvent reservoir filter clean. If the filter becomes plugged,
clean or replace it.
If the Waters Prep system is not used for several weeks, flush the system
(outlet line, inlet lines, and check valves) with filtered, distilled water
containing 10% methanol or acetonitrile. Remove the column if it is not
compatible with this storage protocol.
Warning: Always follow safe laboratory practices when handling
solvents. Know the chemical and physical properties of the solvents.
Refer to the Material Safety Data Sheet for the solvents in use.
Caution: Do not allow the solvent system to exceed 5 psi pressure when
you use pressurized solvent containers.
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