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Waters Extraction+ - User Manual

Waters Extraction+
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USER MANUAL
©2022 Waters Corporation
Extraction+
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Summary

Safety Instructions

Device Usage and Handling

Details prohibited uses, setup location, operating distance, indoor use, maintenance, modifications, SELV circuits, and overheating.

About this User Manual

Manual Usage and Disclaimer

Explains how to use the manual, outlines responsibilities, and notes that information is subject to change without notice.

Product Overview and Setup

Extraction+ Manifold Components

Identifies key components of the Extraction+ manifold, including the collar lifter, labware, and base.

Extraction+ Vacuum Pump Components

Details the components of the Extraction+ vacuum pump, such as LED indicators, inlets, power button, and rear connections.

Getting Started

Install Vacuum Pump and Liquid Trap System

Provides step-by-step instructions for setting up the vacuum pump and waste container, including hose connection.

Manifold Installation

Setup for Cartridge Installation

Outlines the steps for connecting the Extraction+ manifold for cartridge use, including hose and adapter placement.

Setup for Filter Plate Installation

Details the steps for setting up the manifold with filter plates, including the use of manifold spacers.

Manifold Spacers for Filter Plates

Spacer System and Usage Examples

Explains the manifold spacer system, available sizes, and provides examples of optimal usage with different collection plates.

Accessory Usage for Filter Plates and Cartridges

Plate Sealer for Filter Plates

Recommends using a plate-sealer film on unused filter plate cavities for even vacuum application.

Sealing Caps for Cartridges

Suggests using sealing caps in unused cartridge adapter slots for even vacuum application.

Collection Labware Rack for Cartridges

Describes the Collection Labware Rack Domino for holding consumables during extraction phases.

Labware Gripper for Andrew+

Details adding the Microplate Gripper tool for automated movement of labware and racks.

Connection to OneLab

Initial Setup and WiFi Connection

Guides users through connecting the Extraction+ vacuum pump to a power source and establishing a WiFi connection to OneLab.

Web Browser Installation

Instructs users to launch a web browser and navigate to a specific IP address to complete the installation.

Operations with OneLab

Protocol Design

Explains the drag-n-drop editor for designing precise and complete experimental protocols.

Experiment Execution

Details how OneLab selects and uses the Extraction+ for various experiment setups and guides users through preparation.

Starting and Controlling Experiments

Guides users to start experiments and follow visual instructions provided by OneLab for device control.

Technical Data

Extraction+ Pump Specifications

Lists technical specifications for the Extraction+ pump, including pump rate, measurement ranges, dimensions, and power.

Extraction+ Manifold Specifications

Provides technical specifications for the Extraction+ manifold, including compatible labware and dimensions.

Extraction+ Pump Power Management

Details the procedures for powering the Extraction+ pump on and off, including standby mode.

Maintenance

Filter Cartridge Replacement

Provides a step-by-step visual guide for replacing the filter cartridge in the Extraction+ device.

Certifications and End of Life

Unit End of Life Information

Advises contacting Waters for end-of-life directions and information, in accordance with WEEE directive.

Electrical Safety, EMC, and Radio Certifications

Lists certifications for electrical safety, EMC, radio, FCC compliance, and RoHS.

Manufacturer Contact Information

Provides contact details for Waters Corp and Andrew Alliance S.A. for support and inquiries.

Summary

Safety Instructions

Device Usage and Handling

Details prohibited uses, setup location, operating distance, indoor use, maintenance, modifications, SELV circuits, and overheating.

About this User Manual

Manual Usage and Disclaimer

Explains how to use the manual, outlines responsibilities, and notes that information is subject to change without notice.

Product Overview and Setup

Extraction+ Manifold Components

Identifies key components of the Extraction+ manifold, including the collar lifter, labware, and base.

Extraction+ Vacuum Pump Components

Details the components of the Extraction+ vacuum pump, such as LED indicators, inlets, power button, and rear connections.

Getting Started

Install Vacuum Pump and Liquid Trap System

Provides step-by-step instructions for setting up the vacuum pump and waste container, including hose connection.

Manifold Installation

Setup for Cartridge Installation

Outlines the steps for connecting the Extraction+ manifold for cartridge use, including hose and adapter placement.

Setup for Filter Plate Installation

Details the steps for setting up the manifold with filter plates, including the use of manifold spacers.

Manifold Spacers for Filter Plates

Spacer System and Usage Examples

Explains the manifold spacer system, available sizes, and provides examples of optimal usage with different collection plates.

Accessory Usage for Filter Plates and Cartridges

Plate Sealer for Filter Plates

Recommends using a plate-sealer film on unused filter plate cavities for even vacuum application.

Sealing Caps for Cartridges

Suggests using sealing caps in unused cartridge adapter slots for even vacuum application.

Collection Labware Rack for Cartridges

Describes the Collection Labware Rack Domino for holding consumables during extraction phases.

Labware Gripper for Andrew+

Details adding the Microplate Gripper tool for automated movement of labware and racks.

Connection to OneLab

Initial Setup and WiFi Connection

Guides users through connecting the Extraction+ vacuum pump to a power source and establishing a WiFi connection to OneLab.

Web Browser Installation

Instructs users to launch a web browser and navigate to a specific IP address to complete the installation.

Operations with OneLab

Protocol Design

Explains the drag-n-drop editor for designing precise and complete experimental protocols.

Experiment Execution

Details how OneLab selects and uses the Extraction+ for various experiment setups and guides users through preparation.

Starting and Controlling Experiments

Guides users to start experiments and follow visual instructions provided by OneLab for device control.

Technical Data

Extraction+ Pump Specifications

Lists technical specifications for the Extraction+ pump, including pump rate, measurement ranges, dimensions, and power.

Extraction+ Manifold Specifications

Provides technical specifications for the Extraction+ manifold, including compatible labware and dimensions.

Extraction+ Pump Power Management

Details the procedures for powering the Extraction+ pump on and off, including standby mode.

Maintenance

Filter Cartridge Replacement

Provides a step-by-step visual guide for replacing the filter cartridge in the Extraction+ device.

Certifications and End of Life

Unit End of Life Information

Advises contacting Waters for end-of-life directions and information, in accordance with WEEE directive.

Electrical Safety, EMC, and Radio Certifications

Lists certifications for electrical safety, EMC, radio, FCC compliance, and RoHS.

Manufacturer Contact Information

Provides contact details for Waters Corp and Andrew Alliance S.A. for support and inquiries.

Overview

The Waters Extraction+ is a laboratory device designed for solid-phase extraction (SPE) workflows, facilitating sample preparation in analytical chemistry. It consists of two main components: the Extraction+ vacuum pump and the Extraction+ manifold. The system is engineered to integrate seamlessly with the Andrew+ Pipetting Robot, enhancing automation and precision in laboratory processes.

Function Description

The primary function of the Extraction+ system is to perform solid-phase extraction, a sample preparation technique used to separate analytes from a sample matrix. This is achieved by drawing liquid samples through a sorbent material packed in cartridges or filter plates using a vacuum. The vacuum pump creates the necessary negative pressure to pull liquids through the extraction labware, while the manifold holds the labware and directs the flow.

The Extraction+ vacuum pump is a core component that generates and controls the vacuum. It features an LED indicator for status, a vacuum inlet for connection to the manifold, and a power button for operation. The pump is equipped with a built-in computer and a built-in wireless adapter, enabling connectivity and control through the OneLab software platform. This allows for the design and execution of extraction protocols, as well as monitoring of the experiment. The pump connects to a waste container via a silicone hose, collecting the discarded liquids during the extraction process.

The Extraction+ manifold is the interface where the extraction labware is placed. It comprises several parts: a collar lifter, a manifold collar, a manifold base that also serves as a waste collector, and specific adapters for different types of labware. The manifold is designed to accommodate various extraction labware, including columns in adapters or cartridges, and filter plates. It also supports different collection labware, such as tubes in adapters or plates with manifold spacers, allowing for flexibility in experimental setup.

When used with the Andrew+ Pipetting Robot, the Extraction+ vacuum manifold is typically placed at a specific deck location (Domino location 5), ensuring consistent and automated operation. The integration with OneLab allows for the creation of flow-through extraction actions, where users can design precise protocols using a drag-and-drop editor. The software guides the user through the experiment, providing visual instructions and recording each step.

Usage Features

The Extraction+ system offers several features to enhance usability and flexibility in laboratory settings:

  • OneLab Integration: The device is designed to work with the OneLab software, providing a comprehensive platform for designing, executing, and monitoring experiments. Users can create custom protocols using a drag-and-drop interface, defining steps such as conditioning, loading, and washing. OneLab automatically selects the Extraction+ and supports various experiment setups, including manual, semi-automated, and fully automated operations.
  • Flexible Labware Compatibility: The manifold can accommodate different types of extraction labware, including cartridges and filter plates. This versatility allows users to adapt the system to a wide range of SPE applications.
  • Manifold Spacers for Filter Plates: For filter plate applications, the system includes manifold spacers of various sizes (3 mm, 15 mm, 17 mm). These spacers allow for fine-tuning the penetration height of the filter plate tips within the wells of the collection labware, ensuring optimal tip penetration (2-3 mm) for efficient extraction. The spacers can be combined to achieve desired heights.
  • Sealing Options for Even Vacuum:
    • Plate Sealer: When using filter plates, a plate-sealer film can be applied to unused filter plate cavities. This ensures that vacuum is applied evenly across the active cavities, improving extraction efficiency.
    • Sealing Caps: For cartridge-based extractions, sealing caps can be placed in unused slots of the cartridge adapter. Similar to plate sealers, these caps help maintain an even vacuum across the active cartridges.
  • Collection Labware Rack (for Cartridge Use): For automated runs with the Andrew+ robot and cartridges, a Collection Labware Rack Domino is used. This rack holds the collection consumables during the initial phases of SPE (conditioning, loading, washing), streamlining the workflow.
  • Labware Gripper (for Andrew+ Use): To facilitate automated movement of collection labware and racks into and out of the manifold by the Andrew+ robot, a Microplate Gripper tool can be added to the robot's tool rack.
  • Installation Flexibility: The vacuum pump and waste container can be placed on or under the workbench, or on the floor, providing flexibility in laboratory setup. The silicone hoses can be cut to appropriate lengths to suit the specific arrangement.
  • Connectivity: The Extraction+ vacuum pump connects to a computer, tablet, or smartphone via WiFi, allowing for remote control and monitoring through the OneLab platform. It also has an Ethernet port for cable connection to a network.

Maintenance Features

The Extraction+ system is designed with user-friendly maintenance in mind, primarily focusing on the replacement of the filter cartridge:

  • Filter Cartridge Replacement: The device features a maintenance hatch that allows access to the internal filter cartridge. The replacement process is straightforward:
    1. Open the maintenance hatch by pushing down the back of the hatch.
    2. Remove the hatch.
    3. Unplug the filter shell from the filter port.
    4. Unplug the small hose.
    5. Unplug the big hose.
    6. Take a new filter and plug in the big hose first.
    7. Plug in the small hose.
    8. Plug the filter into the filter port.
    9. Place the filter inside the compartment.
    10. Push the small hose to straighten it.
    11. Replace the hatch.
  • Cleaning: The device can be cleaned using a wet tissue or isopropanol, ensuring easy upkeep and hygiene in the laboratory environment.
  • Servicing: With the exception of filter cartridge replacement, device maintenance is exclusively reserved for Waters servicing and support representatives, ensuring that complex repairs or adjustments are handled by trained professionals.
  • Overheat Protection: If the device overheats, it will automatically stop operating. Users are instructed to immediately disconnect the device from its power supply in such an event, which acts as a safety and protective measure for the device.

Waters Extraction+ Specifications

General IconGeneral
Extraction TypeSolid-Phase Extraction (SPE)
DescriptionAutomated solid-phase extraction system
Cartridge Size1 mL, 3 mL, 6 mL
Solvent CompatibilityCompatible with a wide range of solvents
AutomationAutomated SPE processing
Software ControlYes
Automation LevelFull automation
CompatibilityCompatible with various sample matrices
DimensionsVaries depending on configuration
WeightVaries depending on configuration
Power Requirements100-240 V, 50/60 Hz
Flow RateAdjustable flow rates

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