Why is there increased system backpressure during injection using Rheodyne injector in Waters Laboratory Equipment?
Increased system backpressure during injection with a Rheodyne injector in Waters Laboratory Equipment is normal. When the injector is in the Inject position, the sample loop generates additional system backpressure. To decrease the system backpressure, return the injector to the LOAD position after loading the sample.
What to do if the Waters Prep Laboratory Equipment system controller screen is blank?
If the screen on your Waters Laboratory Equipment system controller is blank, the screen saver feature may be active. Press any function key to display the screen.
What to do if my Waters Prep Laboratory Equipment pump keeps shutting down due to exceeding the high-pressure limit?
If your Waters Laboratory Equipment pump shuts down because the high-pressure limit is exceeded, there are several potential causes. It could be due to a high flow rate during injection valve actuation, causing a pressure spike; in this case, decrease the flow rate during injection. Another cause may be a blockage in the system after the transducer; try removing components between the pump and the end of the system to locate and eliminate the blockage. Finally, the column might be plugged, so try cleaning or replacing it.
Why is my Waters Prep pump cavitating?
Pump cavitation in your Waters Laboratory Equipment can occur for several reasons. First, check the solvent reservoirs' position; if they are below the solvent proportioning valve, place them on the bench beside the fluid handling unit. Second, inspect tubing connections and fittings for leaks prior to the inlet check valve, as these can cause air aspiration; eliminate any leaks by tightening the fittings or replacing the ferrules. Lastly, the solvent inlet filter might be restricting flow, so clean or replace the filters.
What to do if Waters Prep Laboratory Equipment pump shuts down because the low-pressure limit is exceeded?
If your Waters Laboratory Equipment pump shuts down because the low-pressure limit has been exceeded, the issue is likely due to leaks, empty reservoirs, or bubbles in the pump head. Eliminate any leaks, fill the reservoirs, and prime the pump to resolve this.
How to fix a grinding or shuddering noise from Waters Laboratory Equipment pump?
If you notice a grinding or shuddering noise from your Waters Laboratory Equipment pump, and the high-pressure shutdown has not been exceeded, it could be due to a blockage in the system before the transducer. Clean the pump head outlet tubing, outlet check valve, and the tubing leading to the transducer. If the problem persists, it may be a mechanical issue.
Why is there a pulsing flow from the waste line of my Waters Prep Laboratory Equipment?
A pulsing flow from the waste line in your Waters Laboratory Equipment is often caused by dirty check valve(s). Clean the check valve(s) to resolve this issue.
What to do if Waters Prep RI detector is leaking internally?
If your Waters Laboratory Equipment RI detector is leaking internally or from the purge port, the cell may be damaged because the maximum flow rate or pressure of the RI cell has been exceeded. Ensure you do not exceed 5 mL/min for 2414, 2410, and 410 Detectors, and do not exceed 100 psi for any Waters RI detector.
Why are peak retention times halved in Waters Prep?
Halved peak retention times in Waters Laboratory Equipment are caused by an incorrect pump head volume entered on the PUMP CONFIGURATION screen. For PrepLC operation with 1000-µL pump heads, enter 1000 for the pump head volume on the PUMP CONFIGURATION screen.
Why can't I enter a flow rate above 150 mL/min on my Waters Laboratory Equipment?
If you are unable to enter a flow rate above 150 mL/min on your Waters Laboratory Equipment, it is because an incorrect pump head volume was entered on the PUMP CONFIGURATION screen. For operation with 1000-µL pump heads, enter 1000 for the pump head volume on the PUMP CONFIGURATION screen.
Describes the features and configurations of the Waters Prep Series HPLC systems.
Outlines the initial steps for using the system, including startup and basic operations.
Guides on navigating and operating the instrument using its front panel controls.
Helps determine the appropriate controller configuration for system use.
Covers initial setup and inspection procedures for the system.
Details the electrical specifications and configurations for the system.
Explains how to connect system components and modules.
Making tubing connections.
Installing columns.
Identifying and resolving system problems.
Tests to isolate problem causes.
Routine maintenance tasks.
Explains hazard symbols.
Explains caution symbol.
General warnings.
Operational parameters.
Environmental ranges.
Lists common spare parts.
Spare parts for 150-mL system.
Importance of clean, particle-free solvents.
Determining solvent miscibility.
Benefits of degassing solvents.
General| Pressure Range | Up to 6000 psi |
|---|---|
| Detector Options | UV/Vis, PDA, ELSD, MS |
| Detection Wavelength Range | 190 to 800 nm |
| Solvent Delivery | Binary or Quaternary |
| Fraction Collection | Yes |
| Software Control | MassLynx, or Empower |
| Software Compatibility | Waters FractionLynx, MassLynx, or Empower |
| Pump Type | Piston pump |
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