2-48 Installing the System
5. Install compression fittings on each end of the tubing as described in
“Liquid line connections” on page 2-21, and tighten the fittings at all
column connectors.
Making detector connections
This section describes connecting the column outlet to the detector in the
PrepLC and Delta-Prep systems for large-scale and small-scale operation.
PrepLC or Delta-Prep system
Small-scale operation with a Waters 2487 Detector and semi-prep or prep flow
cell requires these preparations:
• A 0.009- or 0.020-inch ID tubing and a union to connect the
SMALL-SCALE OUTLET, on the front of the column rack, to the
detector cell inlet
• An appropriate receptacle in which to put the waste line from the
detector
Large-scale operation with a Waters 2487 Detector requires the appropriate
flow cell (see the table titled “Flow cell tubing sizes” on page 2-22) and these
preparations:
• A 0.020-inch ID or 0.040-inch ID tubing to connect the LARGE-SCALE
OUTLET connector to the inlet side of the detector cell
• An appropriate receptacle in which to put the waste line from the
detector
Tip: Minimize bandspreading. Keep the tubing length between the column
outlet and detector as short as possible by placing the detector on the rack’s
center level.
Connecting detector semi-prep cells
The table titled “Flow cell tubing sizes” on page 2-22 lists the tubing sizes with
which the detector’s semi-prep cells are plumbed. Consult it when you connect
to the column outlet.
The type of operation, small-scale or large-scale, can require the sample to
flow on different sides of the detector. When replumbing the column outlet
connection to a detector, make sure you reconfigure the signal source so that it
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