Troubleshooting 3-13
Poor peak resolution
Before addressing problems with peak resolution, ensure that peaks are
eluting at the correct retention time. The most common causes of poor peak
resolution can also cause retention time problems.
If peak retention time is correct, determine if the poor resolution occurs:
• Throughout the chromatogram
• At a single peak pair
In a gradient method, if resolution of early peaks is poor but improves later in
the chromatogram, precolumn band broadening might be the problem. If peak
resolution is poor throughout the chromatogram, post-column band
broadening, or loss of column efficiency, might be the cause.
If one peak in the chromatogram is badly misshapen, it may imply that it
interacts with the column by way of a different chemical mechanism.
Refer to the figure “Peak resolution troubleshooting” on page 3-14 to identify a
possible cause of your peak resolution problem.
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