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Waters 717plus User Manual

Waters 717plus
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Waters 717plus Autosampler
Operator’s Manual
34 Maple Street
Milford, MA 01757
173-02TP, Revision 3

Table of Contents

Questions and Answers:

Waters 717plus Specifications

General IconGeneral
TypeAutosampler
Sample CapacityUp to 120 vials
Flow Rate Range0.001 to 10 mL/min
Pressure RangeUp to 6000 psi
Injection Volume Range1 to 100 µL
Precision±0.5%
Operating Temperature4°C to 40°C
CommunicationRS-232

Summary

Chapter 1 Introduction

1.1 Description

Provides a general overview of the Waters 717plus Autosampler's purpose and function.

1.2 Features

Details the key capabilities and functionalities of the autosampler.

Interaction with Other LC Modules

Explains how the autosampler communicates with other chromatography system components.

IEEE-488 Bus Communication

Describes the system's communication capabilities via the IEEE-488 network.

RS-232 Communications

Details the data transfer interface using RS-232 communication.

Purge and Compression

Describes the system's capabilities for flushing and testing gas content.

1.3 Components and Subsystems

Identifies and describes the main parts and modules of the autosampler unit.

Chapter 2 Installation

2.1 Site Requirements

Specifies the necessary space, environmental conditions, and utilities for installation.

Operating Conditions

Outlines the environmental parameters required for optimal autosampler performance.

Power Requirements

Details the electrical specifications for connecting the autosampler to a power source.

2.2 Unpacking and Inspection

Provides instructions for safely removing the unit from its packaging and checking contents.

2.3 Making the Power Supply Connection

Guides the user through connecting the autosampler to the AC power supply.

2.4 Connecting Fluid and Drain Lines

Explains how to connect the various fluid and waste lines to the autosampler.

2.5 Connecting Communication Cables

Details the procedures for connecting communication cables for system integration.

2.5.1 Making Terminal Strip Connections

Covers the setup and signals for the terminal strip connections for external components.

System Controller Connections

Explains how to connect the autosampler to system controllers for coordinated operation.

Auto Zero

Describes how to enable the auto zero function for accurate pressure readings.

2.5.2 Making IEEE-488 Network Connections

Details the process for establishing network connections using the IEEE-488 bus.

2.5.3 Connecting RS-232 Output to Data Module

Guides on connecting the autosampler to data modules via RS-232 for data transfer.

2.6 Changing the Hardware Configuration

Covers procedures for modifying the autosampler's hardware setup, like cover removal or adding modules.

2.6.1 Removing/Replacing the 717plus Cover

Provides instructions for safely removing and reinstalling the autosampler's outer cover.

2.6.2 Installing the Heater/Cooler

Provides step-by-step instructions for installing the optional heater/cooler module.

2.6.3 Installing the Large Sample Loop

Explains how to install the auxiliary loop for increased sample injection volumes.

Chapter 3 Startup and Configuration

3.1 Powering Up the Autosampler

Guides the user through the initial power-on sequence and system initialization.

3.2 Using the Front Panel

Describes how to navigate and interact with the autosampler using its front panel interface.

3.2.1 Accessing Menus

Explains how to access the main system operations and menus via function keys.

3.2.2 Entering Operating Parameters

Details how to input and modify system parameters using the keypad and menus.

3.3 Flushing the Autosampler

Provides instructions for purging the system to prepare it for operation or maintenance.

3.4 Calibrating the Seal Pak

Outlines the essential steps for calibrating the injector seal pak for accurate performance.

3.5 Priming the Needle Wash Pump

Details the procedure for priming the needle wash system to ensure proper operation.

3.6 Configuring the Autosampler

Covers setting up system parameters to enable communication and define hardware configurations.

3.6.2 Setting Parameters for LC System Operation

Guides on configuring parameters for communication with other LC system components.

Setting Terminal Strip Signal Parameters

Explains how to configure the signal parameters for terminal strip connections.

3.7 System Verification

Describes how to perform system checks to ensure proper functionality after installation or daily use.

3.8 Power Down and Storage

Provides procedures for safely shutting down and storing the autosampler.

Chapter 4 Operating the Autosampler

4.1 Overview of Operation

Gives a high-level summary of the autosampler's operational workflow and features.

4.2 Preparing Samples

Explains the process of preparing samples and selecting appropriate vials for injection.

Selecting a Solvent

Provides guidelines for selecting appropriate solvents for sample preparation and dissolution.

Filtering Samples

Discusses the importance of filtering samples to prevent blockages and maintain system performance.

4.2.2 Selecting Sample Vials

Guides the user on choosing the correct sample vials based on sample and injection parameters.

4.3 Selecting a Needle Wash Solvent

Guides on choosing a suitable solvent for the needle wash system based on sample chemistry.

4.4 Loading the Carousel

Details the steps for correctly loading sample vials into the autosampler carousel.

4.5 Ensuring Reproducibility

Covers essential checks and procedures to maintain consistent and reliable injection performance.

4.5.2 Performing a Purge

Describes how to perform a purge cycle to ensure system readiness and remove air or old mobile phase.

4.5.3 Performing a Compression Check

Explains how to conduct a compression check to test for gas in the mobile phase.

4.6 Running Autosampling

Covers the process of programming, running, and monitoring automated sample injection sequences.

4.6.1 Programming Auto Run

Details how to set up and define parameters for automated sample runs.

4.6.2 Programming Step Functions

Explains how to program specific functions and options for each step in an auto run.

4.6.3 Using Auto Standards

Describes how to program and run auto standards to ensure accuracy during sample analysis.

4.6.4 Using Auto Addition

Explains how to automate the addition of common solutions or reagents to samples.

4.6.5 Using Auto Transfer

Details how to program automated transfer of solutions from vials to sample vials.

4.6.6 Programming the Event Output

Covers programming contact closure outputs to control external devices during a run.

4.6.7 Starting and Stopping an Auto Run

Provides instructions on how to initiate and terminate automated sample runs.

4.7 Making a Stat Run

Explains how to perform priority sample injections that interrupt the ongoing auto run.

4.8 Sampling Considerations

Discusses factors affecting sampling performance and how to adjust configurations accordingly.

4.8.1 Temperature Control

Details how to set and manage the sample compartment temperature using the heater/cooler.

4.8.2 Microbore/Semiprep Injections

Covers physical reconfigurations for performing microbore or semiprep injections.

4.8.3 Injecting Viscous Samples

Explains how to adjust settings for improved injection accuracy with viscous samples.

4.8.4 Purging During an Auto Run

Describes how to enable automatic purging during a run to maintain system integrity.

4.8.5 Using Buffered Mobile Phase

Provides guidelines and precautions for using buffered mobile phases in the autosampler.

Chapter 5 Maintenance

5.1 Defrosting the Heater/Cooler

Guides the user on when and how to perform a defrost cycle for the heater/cooler.

5.2 Replacing the Syringe

Details the procedure for removing the old syringe and installing a new one.

5.3 Replacing the Seal Pak

Covers the process for replacing the injector seal pak and needle assembly.

5.3.1 Removing the Injector

Provides instructions for safely removing the injector assembly from the autosampler.

5.3.2 Removing the Seal Pak

Details the steps for detaching the seal pak from the injector assembly.

5.3.3 Replacing the Needle

Explains how to remove and replace the injector needle.

5.4 Replacing In-Line Filters

Guides on how to replace in-line filters, often done to resolve high backpressure issues.

Chapter 6 Troubleshooting

6.1 Safety and Handling

Outlines critical safety precautions and handling procedures for troubleshooting.

6.2 Error Messages

Describes various error message types and their potential causes.

6.2.1 Informational Error Messages

Explains messages alerting the operator to incorrect parameter entries or operations.

6.2.2 Calibration (CAL) Error Messages

Lists common error messages encountered during seal pak calibration.

6.2.3 Carousel (CAR) Error Messages

Addresses errors related to the carousel, its reader, or drive mechanism.

6.2.5 Injector (INJ) Error Messages

Details error messages specific to the injector's sensors and calibration status.

6.3 Troubleshooting Tables

Provides tables listing symptoms, causes, and corrective actions for common autoinjector problems.

6.4 Troubleshooting Procedures

Outlines specific procedures for diagnosing and resolving issues like valve leaks or manual purges.

6.4.2 Valve Leak Test

Describes how to perform a valve leak test to isolate fluid system problems.

6.4.3 Manual Transducer Adjustment

Guides on manually adjusting the transducer if auto zeroing fails.

Appendix A Specifications

Operational specifications

Lists key performance parameters such as injection volume range, linearity, and precision.

Mechanical specifications

Details physical attributes including wetted materials, dimensions, and number of vials.

Environmental specifications

Outlines operating temperature and humidity ranges for the autosampler.

Power Requirements

Specifies the voltage, frequency, and current requirements for the unit.

Communications

Lists the specifications for IEEE-488, RS-232, and event output connections.

Appendix B Spare Parts

Spare Parts List

Provides a comprehensive list of available spare parts with their corresponding part numbers.

Appendix C Reproducibility and Solvent Degassing

C.1 Factors Affecting Reproducibility

Discusses chemical and instrument factors that impact the consistency of injection results.

C.2 Solvent Degassing

Explains the importance and methods of degassing solvents for improved chromatographic performance.

C.2.2 Solvent Degassing Methods

Describes common techniques like sparging, heating, vacuum, and sonication for solvent degassing.

Appendix D Selecting Sample Vials

Sample Chemistry

Guides selection of vials based on sample chemical properties and compatibility.

Volume of Sample and Injection

Provides data on vial capacities and maximum injection volumes for different vial types.

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