Flushing the Autosampler 59
3
3.3 Flushing the Autosampler
Solvent Considerations
When flushing or purging your 717plus Autosampler, make sure the solvent is properly
degassed and miscible with the previous mobile phase in the autosampler.
Flushing
To flush the 717plus Autosampler:
1. Place the red line in a waste container to catch the solvent.
2. Set the mobile phase pump to deliver 1 mL/min of 100 percent methanol.
3. Continue pumping mobile phase for 10 minutes or until all air is forced out of the
autosampler (no bubbles appear in the waste container).
3.4 Calibrating the Seal Pak
The Waters 717plus Autosampler seal pak must be calibrated upon startup or after
installation or service.
Injector calibration involves:
• Purging the autosampler
• Auto zeroing the transducer
• Calibrating the seal pak
3.4.1 Purging the Autosampler
Before calibrating the seal pak, run a purge to ensure that there is no air in the
autosampler, the mobile phase is fresh, and there are no bubbles in the syringe.
1. Using a degassed solution of 100 percent methanol, set the solvent delivery pump
to 1 mL/min.
2. Remove the syringe cover panel to examine the syringe for bubbles.
3. From the Main Menu, press Edit Purge. The Purge and Compressibility menu
appears (Figure 3-13
), listing the default parameter values.
4. Use the arrow keys to set Compression Check to No, then press Enter. Press Start
Purge.
5. Run at least two purges before calibrating the seal pak.
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