Selecting a Needle Wash Solvent 83
4
To use a needle wash:
1. Select a needle wash solvent based on the chemistry of the mobile phase.
2. Place the green line in the needle wash solvent reservoir and place the reservoir on
the same level as the autosampler (Figure 4-4
).
Note: To avoid siphoning, do not place the needle wash reservoir above bench
height.
For extended storage, replace the needle wash solvent with a recommended
storage solvent (see Section 3.8,
Power Down and Storage).
Table 4-1 Suggested Needle Wash Solvents
Mobile Phase Needle Wash
Buffered aqueous, reverse
phase
50% H
2
O 50% MeOH
Non aqueous, reverse phase 100% MeOH
Normal phase Mobile phase
GPC Mobile phase
Ion exchange Paired ion reagent in H
2
O