Startup and Configuration 62
3
3.4.3 Calibrating the Seal Pak
The autosampler must be calibrated after:
• Initial installation
• Replacing the seal pak and needle
Before Calibrating
Before running the seal pak calibration, ensure that:
• Two purges have occurred
• Pump pressure is 400 to 600 psi and flow at least 0.5 mL/min
• Solvent is 100 percent degassed methanol
• Mobile phase pump is free of pressure fluctuations
• Remove your column if it is sensitive to sudden pressure changes
To calibrate the seal pak:
1. Adjust the pump flow to achieve a pump pressure of between 400 and 600 psi.
Note: To attain sufficient backpressure without a column, it may be necessary to
partially cap the red line with the union and fitting plug assembly included in the
717plus Startup Kit.
2. To access and run the seal pak calibration, press the keys indicated in the next
flowchart (Figure 3-6
).