Operating the Autosampler 82
4
Assembling Sample Vials
Assemble the sample vials as shown in Figure 4-3.
Figure 4-3 Sample Vial Assembly
4.3 Selecting a Needle Wash Solvent
The needle wash prevents cross-contamination of samples by wiping the needle with
porous, wetted frits. Select a needle wash solvent based on the sample and mobile phase
chemistry. Ta b l e 4- 1
lists some suggested needle wash solvents. High sample
concentrations may require stronger needle wash solvents.
STOP
Attention: Do not overtighten the cap with a self-sealing septum. Overtightening
(septum dimples in) may cause the cap to fit incorrectly. Also, the use of self-sealing
septa may cause variable injector volumes on multiple injections due to vacuum buildup.
STOP
Attention: Do not use buffers in the needle wash solvent. Buffers decreases seal life.
Septum
Screw Cap
Snap-on Cap
Limited Volume
Insert
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