Waters HPLC - Chromatography Troubleshooting

3-16 Troubleshooting, Testing, and Maintenance
Column troubleshooting
Chromatography troubleshooting
This section lists symptoms of chromatography problems, possible causes, and
suggested corrective actions.
Column problem
Replace
column inlet
frit
Pressure
reduced?
Determine plate
count to verify
performance
Determine
plate count
Replace
column outlet
frit
Retention time of
marker compound
changed?
Clean/replace
column and
determine plate
count
Pressure
reduced?
Clean/replace
column and
determine plate
count
Has plate
count changed?
Clean/replace
column and
determine plate
count
Call Waters
Technical Service
No
Yes
No
No
Yes
Yes
No
No
Yes
Yes
Has pressure
changed?