Waters HPLC - Page 99

Troubleshooting 3-15
Peak resolution troubleshooting (Continued)
Column
Before using this troubleshooting tree, use those that appear earlier in the
chapter to isolate the column as problem’s cause.
Refer to the figure below to determine the possible cause of your column
problem.
Continued from
previous page
Extra
column band
broadening?
Detector
filter constant
too high?
Adjust filter
constant
Coeluting
peaks?
If retention time
correct, is sample
contaminated or
degraded?
Use fresh
sample and
standard
Call Waters
technical service
No
Yes
No
No
No
Yes
Yes