Tighten the fitting finger-tight, plus as much as an additional 1/6-turn using a 1/4-inch open-end
wrench.
Reinstalled tightening
2.7
Sample preparation
Micro-scale UPLC analysis places some additional restrictions on sample preparation.
Particle-free mobile phase solvents and sample solutions are essential for micro-scale UPLC
analysis. See General guidelines for a microscale system for recommendations on choosing and
handling solvents.
November 26, 2019, 715003588 Revision C
Page 47
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