Zeiss Axiolab 5 - User Manual

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Operating Manual

Axiolab 5

Upright Microscope for Routine and Entry-level Research

Microscope for Routine and Entry-Level Research

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Quick Reference Guide134 pages

Questions and Answers

W
waynesellersAug 11, 2025
What to do if the Zeiss Axiolab 5 Microscope image has shadows or is not entirely visible?
- R
  Robert SmithAug 11, 2025
  Shadows or inhomogeneous image brightness, or a field that is not entirely visible in your Zeiss microscope image, can be due to: * The vis/phot push-pull rod/shift knob on the photo tube is not in the correct functional position (intermediate position). Move the vis/phot push-pull rod/shift knob to the correct functional position (end position). * The nosepiece with objective not engaged in click-stop. Push in nosepiece with objective until it engages. * The condenser is not correctly adjusted. * The aperture diaphragm is not correctly adjusted. * The luminous-field aperture is not correctly adjusted. * The filter has not been inserted correctly in the filter mount. Insert filter correctly in the filter mount.
S
Sharon MedinaOct 26, 2025
Why is my Zeiss Axiolab 5 image blurry and has low contrast?
- M
  Mark RiceOct 26, 2025
  Low resolving power and poor image contrast in your Zeiss microscope can be caused by several factors: * Incorrect adjustment of the aperture diaphragm. Adjust the aperture diaphragm as per 2/3 rule or the specimen features. * The condenser not being correctly focused and front lens 0.9 not swiveled in/out correctly. Focus the condenser and swivel front lens 0.9 in or out correctly. * Using the wrong cover glass thickness for transmitted light objectives. Use a standard cover glass with a thickness of 0.17 mm. * Specimen slide placed upside down. Turn the specimen slide over so the specimen side is on top. * Using the wrong immersion oil, or none at all, with immersion objectives. Use immersion oil 518 N or 518 F from ZEISS. * Air bubbles in the immersion oil. Repeat the oilin...

Summary

Introduction, Safety, and Warranty

Notes on Instrument Safety

Essential safety guidelines, warning symbols, and hazard information for microscope operation.

Warning Labels on Microscopes

Identification and explanation of warning labels affixed to the microscope for user safety.

Warranty Information

Details on manufacturer's warranty, exclusions, and liability limitations for the instrument.

Microscope Description and Features

Intended Use and Technical Specifications

Overview of applications, technical data, dimensions, weight, and ambient conditions.

Microscope Stand Models and Components

Detailed description of various stand models and functional components like tubes, stages, condensers, and accessories.

Microscope Operating Modes and Connectivity

Explains standalone system use, connections via HDMI, Wi-Fi, WLAN, USB, and ZEN software.

Start-Up and Basic Setup

Mounting Standard and Optional Components

Step-by-step instructions for installing and assembling microscope parts like tubes, objectives, stages, and condensers.

Power Connection and Operation

Procedures for connecting the microscope to power, switching it on/off, and using the Light Manager function.

Initial Setup and Adjustments

Guide for setting inter-pupillary distance, viewing height, and adjusting for user's visual impairment (ametropia).

Illumination and Contrast Methods

Transmitted Light Techniques

Detailed procedures for brightfield (Köhler), darkfield, phase contrast, and polarization microscopy.

Conoscopy and Optical Characterization

Methods for conoscopic observation and determining crystal optical character using polarization.

Reflected Light Techniques

Procedures for reflected light brightfield, darkfield, polarization, and fluorescence microscopy.

Care, Maintenance, and Troubleshooting

Instrument Care and Maintenance

Guidelines for cleaning, protecting, and performing basic maintenance on the microscope.

Troubleshooting Common Issues

A comprehensive guide for diagnosing and resolving common microscope and camera operational problems.

Service and Firmware Updates

Information on obtaining service, authorized repairs, and updating the microscope's firmware.

Appendix

List of Abbreviations

An alphabetical compilation of all abbreviations used throughout the manual for easy reference.

Index and System Overview

An alphabetical index for quick topic lookup and a visual system overview diagram.

Zeiss Axiolab 5 Specifications

General

Microscope Type	Upright Microscope
Illumination	LED illumination
Optical System	Infinity Optical System
Eyepieces	10x
Objectives	Plan-Apochromat
Condenser	Abbe condenser
Stage	Mechanical stage
Focusing	Coaxial coarse and fine focusing
Camera Port	Camera port available for digital imaging
Contrast Methods	Brightfield, Darkfield, Phase contrast, Polarization contrast