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Zeiss Axiocam MR User Manual

Zeiss Axiocam MR
89 pages
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Number of this manual: M 60-3-0014 e
Printed 12.2008

Table of Contents

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Questions and Answers:

Zeiss Axiocam MR Specifications

General IconGeneral
Sensor TypeCCD
Resolution1388 x 1040 pixels
Pixel Size6.45 µm x 6.45 µm
InterfaceIEEE 1394 (FireWire)
Bit Depth12-bit
Exposure Time1 ms to 60 s
CoolingThermoelectric cooling (regulated)

Summary

Safety

Disposal and Recycling

IMPORTANT NOTE

FireWire (IEEE 1394 a) Interface

The FireWire (IEEE 1394 a) interface is defined as a "hot plugging" interconnection, i.e. peripherals (cameras, drives, etc.) may be connected or disconnected while the computer is powered up.

1. Installation

1.1 Safety Regulations

Please read this chapter carefully and observe the regulations in order to ensure your safety and the intended operation of the system.

General notes

Please ensure the notes described below are adhered to when setting up and operating the AxioCam MR camera.

Backup copies

We strongly recommend that all users save the data they create, such as images, measurement data, archives, reports, forms and documents, at regular intervals on an external medium.

1.2 System Requirements

The appropriate system requirements relating to hard- and software equipment can be found in the file "Installation Manual.PDF" on the actual product DVD.

Setting up the camera system

The standardized FireWire interface allows the camera to be used for a variety of applications. The following graphic provides an overview of the possible hardware configurations and the required accessories.

Operating the camera on a PC

Depending on your system specifications, you can choose between two forms of connection.

Operating the camera on a notebook

Depending on your notebook's specifications, you can choose between two forms of connection.

Mounting the camera onto the microscope

To mount the camera onto your microscope's TV port, use a 1.0x or a 0.63x C-mount adapter.

Function indicator

After you have switched on the PC or the FireWire hub, the LED on the camera lights up in red for a short time, before changing to green.

Expansion socket

In addition to the socket for the data cable, the camera also has an additional socket. This socket can be used to open and close an external shutter via trigger output signals.

Second FireWire socket

This FireWire socket may be used to cascade FireWire devices. This feature should only be used, if you are sure that the power specification of your computer's FireWire interface fits the needs of all connected devices.

Checking the equipment has been set up correctly

Check the following connections: The AxioCam MR is mounted onto the microscope and is receiving light. The AxioCam MR is connected to the PC or notebook via the FireWire data cable.

Installing the driver software

In order to operate the AxioCam MR the AxioVision software is needed. First connect the AxioCam MR to your PC, and then install the driver software.

Installing the camera driver with the hardware wizard

This section describes installation using the hardware wizard. If you want to update the camera installation or if you have interrupted the driver wizard, follow the instructions in chapter "Installing the camera driver using the setup program".

Installing the camera driver using the setup program

In this section, installation is explained using screenshots from Microsoft Windows XP Professional. After you have inserted the DVD into the DVD drive installation starts automatically.

How to correct the driver entry in the device manager

If the entry in the Device Manager must be corrected, i.e. if a question mark or an exclamation mark is in front of the AxioCam MR entry, proceed as follows.

Resetting the AxioCam MR firmware

If the color of the LED on the back of the AxioCam MR does not immediately change from red to green but goes out after the insertion of the FireWire cable, this means that the AxioCam MR's firmware is not functioning properly.

1.3 Notes on Maintenance

Camera, interface and power supply are maintenance free. The internal optical components of the camera should always be protected.

2. Image Acquisition

2.1 General

The AxioCam MR is a professional, high-resolution digital camera. It has been specially optimized for routine and research applications in the area of light microscopy.

2.2 Quick Guide to the First Image with AxioVision

If you are using several cameras on your system you must always make the AxioCam MR camera the active camera before proceeding.

2.3 Adjust Property Page

This property page contains a summary of the most important camera settings, which are constantly used during operation.

Exposure

The camera's exposure time can be adjusted to the lighting conditions on the microscope using a slider or by entering an exposure time directly into the input field.

White balance

In order to achieve a color display which is neutral and close to reality it is necessary to balance the individual color channels against one another.

Color adjustment

On a microscope, however, the brightness of a halogen lamp is often adjusted by changing the lamp voltage. This changes the color of the light emitted at the same time, though.

Color saturation

Using the Saturation slider, it is possible to increase or decrease the color intensity of the acquired images.

Histogram

A histogram indicates the intensity distribution of the image signal in the live image and the acquired image. It provides a clear representation of the brightness distribution in the current image.

2.4 Frame Property Page

The Frame property page can be used to select a frame for the image acquisition and is splitted in the fields Camera Mode and Frame.

Camera mode

RGB and B/W: Two types of AxioCam MR are produced; one with a CCD sensor with a color mask on the chip (AxioCam MRc), and the other with a black and white sensor (AxioCam MRm).

Resolution

The camera resolution can be selected between Binning and Standard. The camera has a 2/3" CCD sensor with dimensions of 8.8 mm x 6.6 mm.

Binning

The process of Binning ensures that the information in neighboring pixels, in both a horizontal and vertical direction, is summarized on the camera's sensor.

Size of the resulting files

The size of the resulting files depends on the choice of resolution and gray value scaling.

Frame

In this field you can specify whether the frame or the entire area of the sensor is used for the acquisition.

2.5 General Property Page

On this property page you enter the basic settings for the camera.

Digital gain

Using the slider it is possible to change the interpretation of the brightness of the image data received. This allows you to display dark images as very bright images.

Image orientation

An AxioCam MR image is adjusted in such a way that the image orientation corresponds to the image in the eyepiece when mounted on an upright microscope.

Black reference

Setting a black reference ensures that the image background retains a uniform darkness, even with exposure times in excess of several seconds.

Shading correction

Activate this function if you notice, e.g. with bright-field images, a reduction in image brightness, caused by the optics, at the image edge.

Sharpening

With this function, it is possible for images to receive further processing using a sharpness algorithm immediately after acquisition by the camera.

Shutter control

Via a supplementary cable the AxioCam MR offers a TTL control pulse, which can be used, for example, to control a mechanical shutter.

2.6 Practical Notes on Operation in AxioVision

Below you will find a few practical notes on the speed of the live image, color adjustment, working with exposure times, fluorescence images and configurable toolbars.

Reset camera to a default state

To reset the numerous camera parameters to a reproducible default state, reset functions are available.

Speed of the live image

There are three speed modes available for the AxioCam MR live image. If you use the pointer to click on the Slow icon at the bottom edge of the live image, a pop-up menu with the possible setting options appears.

Notes on optimum color reproduction in AxioVision

Below you will find a detailed description of how best to set up AxioVision to achieve optimum color reproduction with the AxioCam MR.

2.7 Information for Displaying 12 Bit AxioCam Images in AxioVision

A PC graphics card cannot display more than 3 x 8 bits per pixel. However, as we have already mentioned, the AxioCam MR is able to generate 3 x 12 bits per pixel.

2.8 Background Information

Gamma setting. This setting is required to compensate for the inherently non-linear behavior of the image tube ("gamma"). It also influences the quality of the color display.

3. Trouble Shooting

3.1 Software

Please also pay attention to all the comments in the file ReadMe.TXT, which is displayed during the driver installation.

AxioCam MR does not appear in the menu of selectable cameras

Make sure that the AxioCam MR files have been installed in accordance with the installation of the software.

No camera image on your screen

Check the light setting of the microscope. Is the green control light on the camera on? If not, check the cable connections between camera and computer.

The color of the image is green and not realistic coloring

Check white balance and, if necessary, repeat white balance.

3.2 Image Quality

Sudden jolt, vibration or moving objects. If the microscope is jolted whilst you are taking a shot, the image will show periodic interference.

Sudden jolt, vibration or moving objects

If the microscope is jolted whilst you are taking a shot, the image will show periodic interference.

Fluctuations in illumination

If the light source has a fluctuating quality of light intensity, stripes or blotches will show up in the image.

4. Technical Data

4.1 AxioCam MRc (color version)

Sensor: Sony ICX 285, progressive readout, with RGB filter mask. CCD basic resolution: 1388 x 1040 = 1.4 megapixels.

4.2 AxioCam MRm (monochrome version)

Sensor: Sony ICX 285, progressive readout, without filter mask. CCD basic resolution: 1388 x 1040 = 1.4 megapixels.

4.3 Spectral Sensitivity

Normalized to maximum amplitude. Spectral Sensitivity AxioCam HRc/MRc Rev 2.x, incl. BG 40 IR Filter und AxioCam HRm/MRm Rev 2.x, without IR Filter.

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