Zeiss LSM 880 User Guide

880 Table of Contents
880 User guide overview………………………………………………...2-9
Setting up configurations on your own…………………………...10-11
Time Series………………………………………………………………………15
Z-Stacks……………………………………………………………………………16-17
Calibration for Airyscan…………………………………………………….22-25
Acquisition mode- Recommended settings………………........12
Tile Scans and Stitching…………………………………………………...13-14
Adding a DIC Transmitted Light Image……………………………..18
Kohler Illumination…………………………………………………………..19
Incubation………………………………………………………………………..20-21
1

Zeiss LSM 880 Specifications

General

Microscope Type	Confocal Laser Scanning Microscope
Laser Lines	405 nm, 488 nm, 561 nm
Detectors	PMT, GaAsP
Temporal Resolution	Dependent on scanning speed and image size (up to 8 frames per second at 512x512 pixels)
Scanning Method	Galvanometric scanner
Software	ZEN
Superresolution	Airyscan
Dimensions	Variable depending on configuration
Weight	Variable depending on configuration
Objective Lenses	Various objectives available (5x to 100x, air, water, oil immersion)