Chapter 1 FLM300 Overview
9
Fluorophos® ALP Test System Model FLM300 Service Manual
1.3 Calibration
NOTE: Calibrating the FLM300 should be done after
repairs are performed and parts are replaced, or
when the positive control does not test within
specifications.
The ALP Calibrator Set contains Calibrator A, Calibrator
B, and Calibrator C. Readings are performed in duplicate,
therefore, two cuvettes in the heating block should be
labeled for each calibrator as good laboratory practice
(for example; Calibrator A1, Calibrator A2, for a total of
six cuvettes). The procedure is as follows:
1. Dispense 2.0 mL of each calibrator into the proper
cuvette. Place the six cuvettes into the cuvette heating
block to warm at 38°C for thirty minutes.
2. Dispense 75 uL of the product sample into each
cuvette and mix thoroughly.
3. Press CALIB. Press the < or > button to choose “Milks,”
“Cheeses,” or “other” product category, then press
ENTER. Then press the < or > button or use the
numeric keypad to select the correct product channel
(for example; Whole Milk, Low Fat 1%, Low Fat 2%,
etc.) and press ENTER.
4. When the display reads “Insert A STD: [START]”, place
Calibrator A1 into the cuvette holder, close the door,
and press START. The FLM300 reads Calibrator A1,
and after 30 seconds it will display “Insert A STD:
[START]” to prompt for Calibrator A2.
5. Remove the first cuvette and place the second cuvette
in the cuvette holder. Close the door and press START.
If both A Calibrators are within specifications, the
FLM300 will display “Insert B STD: [START]”.
6. Calibrators B and C follow the same instructions as
Calibrator A. The FLM300 will prompt for each sample.
When the FLM300 finishes reading the two Calibrator
C cuvettes, the display will read “Calibration
Complete”.
7. If one set of calibrators is not within product
specifications, the FLM300 will not advance to the
next calibration level. If this happens, prepare new
calibrators and repeat calibration from the beginning.
8. If, after the FLM300 has finished reading the C
Calibrator, the display reads “Unacceptable Calib.
Ratio.” Refer to “Error Messages” on page15.
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