6 Reference
Negative Ion Mode Checkout Conditions
110 Agilent 6400 Series Triple Quad LC/MS System Installation Guide
Tabl e 1 8 MS
ESI (6420/
6430/6460)
AJS (6460) AJS (6470) AJS (6490) AJS (6495)
Capillary 3000 V 2500 V 2500 V 3000 V 3000 V
Collision Energy 13 V14 V16 V14 V14 V
Fragmentor100 V130 V130 V380 V (fixed)380 V (fixed)
Accelerator
voltage
7 V3 V3 V3 V3 V
Delta EMV 200 V200 V200 V200 V200 V
Time Filtering 0.03 min 0.03 min 0.03 min 0.03 min 0.03 min
Compound chloramphenicol chloramphenicol chloramphenicol chloramphenicol chloramphenicol
Scan MRM MRM MRM MRM MRM
MS1 Res Unit Unit Unit Unit Unit
MS2 Res Unit Unit Unit Unit Unit
Precursor Ion 321.2 m/z 321.2 m/z 321.2 m/z 321.2 m/z 321.2 m/z
Product Ion 152.0 m/z 152.0 m/z 152.0 m/z 152.0 m/z 152.0 m/z
Dwell 200 ms 200 ms 200 ms 200 ms 200 ms
Low Pressure
Funnel RF
60 V 60 V
High Pressure
Funnel RF
150 V 150 V
Sample (stock) chloramphenicol,
5ng/µL
chloramphenicol,
5ng/µL
chloramphenicol,
5ng/µL
chloramphenicol,
5ng/µL
chloramphenicol,
5ng/µL
Concentration
after dilution
500 fg/µL 500 fg/µL 500 fg/µL 500 fg/µL 500 fg/µL
Dilution solvent 70:30
water:methanol
70:30
water:methanol
70:30
water:methanol
70:30
water:methanol
70:30
water:methanol