Agilent Technologies 6495B User Manual

Reference 6
Negative Ion Mode Checkout Conditions
Agilent 6400 Series Triple Quad LC/MS System Installation Guide 109
Negative Ion Mode Checkout Conditions
These checkout conditions are used in Negative Ion Mode checkout.
Tabl e 1 5 HPLC Pump
Time %A (water) %B (Methanol) Flow
Initial90100.5 mL/min
0.190100.5 mL/min
12980.5 mL/min
22980.5 mL/min
2.01 90 10 0.5 mL/min
3.7 Stop
Tabl e 1 6 HPLC Autosampler
Injection volume 2 µL
Autosampler temperature 4°C
Needle wash flushport 75:25 methanol:water
Needle wash location Flushport, 10 seconds
Tabl e 1 7 Source
ESI (6420/
6430/6460)
AJS (6460) AJS (6470) AJS (6490) AJS (6495)
Gas Temp 310°C 200°C 200°C 210°C 210°C
Gas Flow 13 L/min 6 L/min 6 L/min 17 L/min 17 L/min
Nebulizer 25 psi 35 psi 35 psi 25 psi 25 psi
Sheath Gas Temp 400°C 400°C 400°C 400°C
Sheath Gas Flow 12 L/min 12 L/min 12 L/min 12 L/min
Nozzle Voltage 1000 V 1000 V 2000 V 2000 V

Agilent Technologies 6495B Specifications

General

Brand	Agilent Technologies
Model	6495B
Category	Analytical Instruments
Language	English