Installation Completion and System Verification 3
Step 3. Prepare the performance evaluation samples
Agilent 6400 Series Triple Quad LC/MS System Installation Guide 59
3 Prepare the second dilution (500 fg/μL) chloramphenicol:
a Use a clean graduated pipette to transfer 1 mL of the 100 pg/μL
chloramphenicol dilution to a pre-rinsed 100 mL volumetric flask.
b Dilute to the 100 mL mark with the 70:30 water:methanol solution.
c Use 5 to 10 mL of this solution to rinse one of the plastic storage bottles
and fill it with the remaining solution.
d Label this bottle “500 fg/μL chloramphenicol” along with the preparation
date.
This provides 500 fg/μL at 2 μL injection volume, which equals the final
1 pg chloramphenicol amount that is needed for performance verification.
Positive Mode (for all 6400 Series LC/MS)
Required Parts • 1 mL graduated pipette (p/n 9301-1423)
• 2 × 100 mL volumetric flasks (p/n 9301-1344)
• ES/APCI Positive Ion Performance Standard (p/n G2423A)
• Plastic bottle for storing first dilution (p/n 9301-1433)
1 Prepare 2 liters of a 70:30 mixture of acetonitrile:water.
2 Prepare the first dilution (50 pg/μL) reserpine:
a Use a clean graduated pipette to transfer 1.0 mL of the 5 ng/μL reserpine
(p/n G2423A) standard to a pre-rinsed 100 mL volumetric flask.
b Dilute to the 100 mL mark with the 70:30 mixture of acetonitrile:water
solution that was prepared in step 1. Invert the flask at least 10 times to
ensure proper mixing of the checkout sample.
c Use 5 to 10 mL of this solution to rinse one of the plastic storage bottles
and fill it with the remaining solution.
d Label this bottle “50 pg/μL reserpine” along with the preparation date.
3 Prepare the second dilution (500 fg/μL) reserpine:
a a Use a clean graduated pipette to transfer 1 mL of the 100 pg/μL
reserpine dilution to a pre-rinsed 100 mL volumetric flask.
b Dilute to the 100 mL mark with the 70:30 mixture of acetonitrile:water
solution.
c Use 5 to 10 mL of this solution to rinse one of the plastic storage bottles
and fill it with the remaining solution.
To Next Page
Loading...
Need help?
General
