ENGLISH
Tips for Getting the Best Chromatographic Results
• Optimize your instrument by minimizing tubing lengths
between components to reduce extra column volume and
band broadening. Use 0.12 mm id red tubing for Fast LC/high
efficiency columns. Learn about capillary options at
www.agilent.com/chem/lccapillaries
• Ensure the data collection rate is optimized for your column.
Use a higher collection rate for Fast LC columns (ZORBAX RRHD).
• Use sample filtration or other sample prep as appropriate for your
sample. Learn more at www.agilent.com/chem/sampleprep
• Use certified lamps in your LC instruments for best performance.
Storage Recommendations
(continued)
To protect equipment, it is desirable to remove salts from the
instrument and column by purging the column with the same
mobile phase without the buffer (e.g. using 60:40 ACN/H
2
O to
remove a 60:40 ACN/0.02 M phosphate buffered mobile phase).
Re-equilibration is rapid with the original mobile phase when using
this approach and any possibility of corrosion from the salts is
eliminated.
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