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Cary 8454
Agilent Technologies Cary 8454 User Manual
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Agilent Cary 8454
UV
-
V
isible Spectroscopy
System
Operator’
s Manual
2
Table of Contents
Default Chapter
2
Safety Notices
2
Table of Contents
5
1 Safety Information
9
Safety Information
10
Verifying Safe State
10
Warning Symbols
10
Precautions
12
Solvent Information
13
Flow Cells
13
Solvents
13
Other Precautions
15
Protective Earth
15
Disconnecting Device
15
Allowing Hot Instrument to Cool
16
Environmental Conditions and Power Requirements
17
Pollution Level
18
Regulatory Information
18
Electromagnetic Compatibility
18
2 Introduction to Your System
21
Agilent Cary 8454 UV-Visible Spectrophotometer - Overview
22
Optical System Overview
22
Spectrophotometer Description
26
General Purpose Agilent Chemstation Software for UV-Visible Spectroscopy - Overview
30
User Interface Elements
31
Software Structure
35
Standard Mode Tasks
37
Standard Mode Data Processing
40
3 Installation and Start up
47
Installation Summary for Your General Purpose UV-Visible System
48
General
48
Spectrophotometer
48
Starting a Measurement Session
50
4 Good Measurement Practices
51
General Considerations
52
Spectrophotometer Design
52
Making Measurements
52
Sample Cell Material
53
Optical Specifications of Cells
54
Apertured Cells
55
Flow Cells
56
Handling and Maintaining Cells
57
Solvents
59
Sample Preparation
60
Photosensitive Samples
61
Stirring and Temperature Control
62
Checklist for Best Results
62
Inserting a Cell
65
5 Using Your UV-Visible Spectroscopy System
67
Starting Your First Measurement Session
68
Starting Your UV-Visible Software
70
Measuring Caffeine Absorbance at 273 Nm
71
Saving Your Parameters as a Method
74
Retrieving and Printing a Method
76
Saving and Retrieving Data
79
Saving Your Samples
79
Saving a Selected Spectrum
81
Retrieving Spectra
83
Deleting Current Spectra
84
Print Preview of Reports
85
Finding the Caffeine Absorbance Maximum
88
Entering Your Cell's Path Length
92
Controlling Your Sipper System
93
Using Your Multicell Transport
95
Quantitative Analysis Using a Calibration with Standards
98
Setup
99
Calibration
101
Analysis
103
How Can I be Sure that My Spectrophotometer Works Properly
105
Spectrophotometer Self Test
105
What Maintenance Does My Spectrophotometer Require
107
Cleaning
107
Spare Parts
107
How Can I Get a Deeper Understanding of UV-Visible Spectroscopy
108
When Do I Have to Measure a Blank
110
Index
111
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Agilent Technologies Cary 8454 Specifications
General
Brand
Agilent Technologies
Model
Cary 8454
Category
Laboratory Equipment
Language
English
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