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Agilent Technologies Cary 8454 - User Manual

Agilent Technologies Cary 8454
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Agilent Cary 8454
UV-Visible Spectroscopy
System
Operator’s Manual

Table of Contents

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Agilent Technologies Cary 8454 Specifications

General IconGeneral
TypeUV-Vis Spectrophotometer
Wavelength Range190 - 1100 nm
Wavelength Accuracy± 0.5 nm
Photometric Range-4 to 4 Abs
Light SourcePulsed Xenon Flash Lamp
Stray Light< 0.05% at 220 nm
Detector TypePhotodiode
InterfaceUSB
Power Requirements100-240 VAC, 50/60 Hz

Summary

Safety Information

Verifying Safe State

Ensures the instrument is in a safe state after maintenance or service procedures.

Warning Symbols

Explains symbols on the instrument that denote hazards to avoid personal injury or death.

Precautions

Provides essential safety precautions for operating, adjusting, and maintaining the instrument.

Installation and Start Up

Installation Summary

Summarizes key installation steps for the spectrophotometer and PC setup.

Starting a Measurement Session

Guides through the initial steps to start a measurement session, including PC and instrument setup.

Good Measurement Practices

Sample Cell Types and Specifications

Covers material requirements, optical specifications, apertured cells, and flow cells.

Using your UV-Visible Spectroscopy System

Starting Your First Measurement Session

Guides through the initial steps to start a measurement session, including PC and instrument setup.

Saving Your Parameters as a Method

Explains how to save current settings as a reusable method for routine laboratory work.

Entering Your Cells Path Length

Explains how to set the cell path length parameter for accurate quantitative calculations.

Quantitative Analysis using Calibration with Standards

Explains quantitative analysis based on calibration curves derived from standards.

Spectrophotometer Self Test

Performs a quick check of instrument performance without external standards.

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