Agilent Technologies Cary 8454 - Measuring Caffeine Absorbance at 273 Nm

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Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 71

Using your UV-Visible Spectroscopy System 5

Measuring Caffeine Absorbance at 273 nm

This section describes how to measure the caffeine sample that was shipped

with your spectrophotometer. Measurement of this caffeine sample is also

used for the IQ (installation qualification) of your spectrophotometer.

1 Make sure that you are in Standard mode. The mode is indicated on the

toolbar of your Agilent ChemStation session.

2 Select the Fixed Wavelength task in the analysis panel’s selection box.

3 Click Setup in the analysis panel to open the parameter dialog.

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