56 Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual
4 Good Measurement Practices
General Considerations
Flow Cells
We recommend a sipper system with a flow cell for obtaining high precision
measurements. Using a flow cell eliminates the necessity of moving the cell
between blank measurement and sample measurement. Also, the cell can be
rinsed thoroughly with the solution to be measured.
The design of the flow cell should minimize entrapment of bubbles and flow
channeling to provide the most reliable results.
Figure 6 Cells you should not use with the instrument
Quartz cells with transparent apertures,
fluorescence cells, plastic cells