Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 103
Using your UV-Visible Spectroscopy System 5
Quantitative Analysis using a Calibration with Standards
Analysis
1 Remember the orientation of the cell in the cell holder. Lift the level to
release the cell, remove it and flush it three times with about 1 ml of your
caffeine sample (1:1 dilution with distilled water). Then fill the cell with
about 3 ml of the caffeine sample. Make sure that the cell windows are clean
and reposition the cell in the same orientation as for the standard
measurement. Close the cell holder’s level.
2 Press the Sample button on the front of your spectrophotometer or click
Sample on the Instrument Panel to start the measurement.
3 Enter your sample’s information in the Sample Information dialog box and
click OK. The view switches to the samples and your quantitative results
will be displayed with the Sample/Result table.
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