Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual 101
Using your UV-Visible Spectroscopy System 5
Quantitative Analysis using a Calibration with Standards
Calibration
1 Fill your 1-cm path-length quartz cell with distilled water. Lift the lever at
the left side of your cell holder. Put the cell in the cell holder and make sure
the transparent windows face towards the front and the back of the
spectrophotometer. Push the lever down to secure your cell in the cell
holder.
2 Press the Blank button on the front of the spectrophotometer or click Blank
on the Instrument Panel to start the measurement.
3 Remember the orientation of the cell in the cell holder. Lift the level to
release the cell, remove it and flush it three times with about 1 ml of your
caffeine sample. Then fill the cell with about 3 ml of the caffeine sample.
Make sure that the cell windows are clean and reposition the cell in the
same orientation as for the reference measurement. Close the cell holder’s
level.
4 Press the Standard button on the front of your spectrophotometer, or click
Standard on the Instrument Panel to start the measurement.
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