98 Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual
5 Using your UV-Visible Spectroscopy System
Quantitative Analysis using a Calibration with Standards
Quantitative Analysis using a Calibration with Standards
Your quantitative analysis task is based on a calibration with standards. After
a successful calibration the measured standards can become part of your
method. Such a method can be used directly for quantitative analysis of
samples.
After having set up your method, calibrated samples can be analyzed. Several
views of both your standards and the calibration, as well as of your samples
and results are available.
As a quick introduction, a calibration using Beer’s law with a single standard
and the analysis of a sample is described. The only limitation on the number of
samples and standards is the memory capacity of your Agilent ChemStation.
For the practical experiment we use the IQ caffeine sample as standard and a
1:1 dilution with distilled water as sample. For the calibration we use
absorbance data at 273 nm.
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