Agilent InfinityLab LC Series Diode Array Detectors User Manual 84
5 Optimizing the Detector
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion
As a rule-of-thumb the flow cell volume should be about 1/3 of the peak volume
at half height. To determine the volume of your peaks, take the peak width as
reported in the integration results multiply it by the flow rate and divide it by 3).
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the Agilent detector it is necessary to have more precise information
on path lengths of the detector flow cells.
NOTE
This may result in problems when the used peak width is set to large and all
peaks are filtered accordingly.
Part Number Path Length
Cell Volume ()
G4212-60008/G5615-60018 1.0 cm 1.0 µL
G4212-60007/G5615-60017 6.0 cm 4.0 µL
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